지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 419 - 427 (9page)
이용수
초록· 키워드
오류제보하기
Background: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown.
Methods: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay. In planta transformation in heterologous Arabidopsis was generated by floral dipping using Agrobacterium tumefaciens (C58C1). Protein localization was analyzed by confocal microscopy via fluorescent protein tagging. Results: PgUGT72AL1 was highly expressed in the rhizome, upper root, and youngest leaf compared with the other organs. GUS staining of the promoter: GUS fusion revealed high expression in different organs, including axillary leaf branch. Overexpression of PgUGT72AL1 resulted in a fused organ in the axillary leaf branch.
Conclusion: PgUGT72AL1, which is phylogenetically close to PgUGT71A27, is involved in the production of ginsenoside compound K. Considering that compound K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of ginsenosides in ginseng plant growth and development. The organ fusion phenotype could be caused by the defective growth of cells in the boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, and requires further analysis.
Methods: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay. In planta transformation in heterologous Arabidopsis was generated by floral dipping using Agrobacterium tumefaciens (C58C1). Protein localization was analyzed by confocal microscopy via fluorescent protein tagging. Results: PgUGT72AL1 was highly expressed in the rhizome, upper root, and youngest leaf compared with the other organs. GUS staining of the promoter: GUS fusion revealed high expression in different organs, including axillary leaf branch. Overexpression of PgUGT72AL1 resulted in a fused organ in the axillary leaf branch.
Conclusion: PgUGT72AL1, which is phylogenetically close to PgUGT71A27, is involved in the production of ginsenoside compound K. Considering that compound K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of ginsenosides in ginseng plant growth and development. The organ fusion phenotype could be caused by the defective growth of cells in the boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, and requires further analysis.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (36)
참고문헌 신청
[단행본] - Gershenzon J / 2010 / Plant physiology / Sinauer Associates : 369 ~ 400
[학술지(정기간행물)] - Lairson LL / 2008 / Glycosyltransferases: structures, functions, and mechanisms / Annu Rev Biochem 77 : 521 ~ 555
[학술지(정기간행물)] - Campbell JA / 1997 / A classification of nucleotidediphospho-sugar glycosyltransferases based on amino acid sequence similarities / Biochem J 326 : 929 ~ 939
[학술지(정기간행물)] - Coutinho PM / 2003 / An evolving hierarchical family classification for glycosyltransferases / J Mol Biol 328 : 307 ~ 317
[학술지(정기간행물)] - Mackenzie PI / 1997 / The UDP glycosyltransferase gene superfamily : recommended nomenclature update based on evolutionary divergence / Pharmacogenetics 7 : 255 ~ 269
[학술지(정기간행물)] - Lairson LL / 2008 / Glycosyltransferases: structures, functions, and mechanisms / Annu Rev Biochem 77 : 521 ~ 555
[학술지(정기간행물)] - Campbell JA / 1997 / A classification of nucleotidediphospho-sugar glycosyltransferases based on amino acid sequence similarities / Biochem J 326 : 929 ~ 939
[학술지(정기간행물)] - Coutinho PM / 2003 / An evolving hierarchical family classification for glycosyltransferases / J Mol Biol 328 : 307 ~ 317
[학술지(정기간행물)] - Mackenzie PI / 1997 / The UDP glycosyltransferase gene superfamily : recommended nomenclature update based on evolutionary divergence / Pharmacogenetics 7 : 255 ~ 269
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UCI(KEPA) : I410-ECN-0101-2018-524-001599155