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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.44 No.3
- 발행연도
- 2017.9
- 수록면
- 318 - 324 (7page)
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초록· 키워드
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With the development of next-generation sequencing (NGS), a cutting-edge technology, genotype-by-sequencing (GBS) became available at a low cost per sample. GBS makes it possible to customize the process of library preparation to obtain high-quality single nucleotide polymorphisms (SNPs) in the most efficient way. However, a GBS library is hard to construct due to fine-tuning of concentration of each reagent and set-up. The major reason for this is the presence of undigested genomic DNA (gDNA) owing to the efficiency of different restriction enzymes for different species with unknown reasons. Therefore, this proof-concept study is to demonstrate the unpredictable patterns of enzyme digestion from various plants in order to make the reader aware of the caution needed when choosing restriction enzymes for their GBS library preparations. Indeed, no pattern was found for the digestibility of gDNA samples and restriction enzymes in the current study. We suggest that more data should be accumulated on this matter to help researchers who want to apply GBS technologies in a variety of genetic approaches.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
References
참고문헌 (16)
참고문헌 신청
[학술지(정기간행물)] - Arabidopsis Genome Initiative / 2000 / Analysis of the genome sequence of the flowering plant Arabidopsis thaliana / Nature 408 : 796 ~ 815
[학술지(정기간행물)] - Comb M / 1990 / CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2 / Nucleic Acids Research 18 : 3975 ~ 3982
[학술지(정기간행물)] - Davey JW / 2011 / Genome-wide genetic marker discovery and genotyping using next-generation sequencing / Nature Reviews Genetics 12 : 499 ~ 510
[학술지(정기간행물)] - Doyle J / 1987 / Genomic plant DNA preparation from fresh tissue-CTAB method / Phytochemical Bulletin 19 : 11 ~ 15
[학술지(정기간행물)] - Elshire RJ / 2011 / A robust, simple genotypingby-sequencing (GBS) approach for high diversity species / PloS ONE 6 : e19379 ~
[학술지(정기간행물)] - Comb M / 1990 / CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2 / Nucleic Acids Research 18 : 3975 ~ 3982
[학술지(정기간행물)] - Davey JW / 2011 / Genome-wide genetic marker discovery and genotyping using next-generation sequencing / Nature Reviews Genetics 12 : 499 ~ 510
[학술지(정기간행물)] - Doyle J / 1987 / Genomic plant DNA preparation from fresh tissue-CTAB method / Phytochemical Bulletin 19 : 11 ~ 15
[학술지(정기간행물)] - Elshire RJ / 2011 / A robust, simple genotypingby-sequencing (GBS) approach for high diversity species / PloS ONE 6 : e19379 ~
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UCI(KEPA) : I410-ECN-0101-2018-520-001344263