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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.9
- 수록면
- 592 - 603 (12page)
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초록· 키워드
오류제보하기
Purpose: The effects of various concentrations of ascorbic acid on stem cell spheroids derived from intraoral areas are not known yet. Thus, the purpose of this study is to evaluate the effects of different concentrations of ascorbic acid on the morphology and cellular viability of stem cell spheroids derived from the gingival tissues.
Materials and Methods: Stem cells were plated onto silicon elastomer-based concave microwells and grown in the presence of ascorbic acid at concentrations ranging from 0.003% to 0.3%. The morphology of the cells was viewed under an inverted microscope at day 1, 2, 3 and 5. Qualitative live/dead assay and quantitative cellular viability using Cell Counting Kit-8 were performed on day 2 and day 5.
Results: Gingiva-derived stem cells formed spheroids irrespective of ascorbic acid concentration in silicon elastomer-based concave microwells. Increase in the diameter of spheroid were seen with higher concentrations of ascorbic acid. Higher cellular viability was seen in higher concentrations of ascorbic acid.
Conclusion: Within the experimental setting, the application of ascorbic acid on stem-cell spheroids produced an increase in the size and higher viability with higher dosage. It can be suggested ascorbic acid be applied with stem cell spheroids for tissue engineering purposes.
Materials and Methods: Stem cells were plated onto silicon elastomer-based concave microwells and grown in the presence of ascorbic acid at concentrations ranging from 0.003% to 0.3%. The morphology of the cells was viewed under an inverted microscope at day 1, 2, 3 and 5. Qualitative live/dead assay and quantitative cellular viability using Cell Counting Kit-8 were performed on day 2 and day 5.
Results: Gingiva-derived stem cells formed spheroids irrespective of ascorbic acid concentration in silicon elastomer-based concave microwells. Increase in the diameter of spheroid were seen with higher concentrations of ascorbic acid. Higher cellular viability was seen in higher concentrations of ascorbic acid.
Conclusion: Within the experimental setting, the application of ascorbic acid on stem-cell spheroids produced an increase in the size and higher viability with higher dosage. It can be suggested ascorbic acid be applied with stem cell spheroids for tissue engineering purposes.
목차
ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
Ⅲ. RESULTS
Ⅳ. DISCUSSION
Ⅴ. CONCLUSION
참고문헌
참고문헌 (33)
참고문헌 신청
[학술지(정기간행물)] - Kim DO / 2002 / Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals / J Agric Food Chem 50 (13) : 3713 ~ 3717
[학술지(정기간행물)] - Rahman F / 2014 / Ascorbic acid is a dose-dependent inhibitor of adipocyte differentiation, probably by reducing cAMP pool / Front Cell Dev Biol 2 : 29 ~
[학술지(정기간행물)] - Sorice A / 2014 / Ascorbic acid: its role in immune system and chronic inflammation diseases / Mini Rev Med Chem 14 (5) : 444 ~ 452
[학술지(정기간행물)] - Bordignon B / 2013 / Ascorbic acid derivatives as a new class of antiproliferative molecules / Cancer Lett 338 (2) : 317 ~ 327
[학술지(정기간행물)] - Kawada H / 2013 / High concentrations of L-ascorbic acid specifically inhibit the growth of human leukemic cells via downregulation of HIF-1alpha transcription / PLoS One 8 (4) : e62717 ~
[학술지(정기간행물)] - Rahman F / 2014 / Ascorbic acid is a dose-dependent inhibitor of adipocyte differentiation, probably by reducing cAMP pool / Front Cell Dev Biol 2 : 29 ~
[학술지(정기간행물)] - Sorice A / 2014 / Ascorbic acid: its role in immune system and chronic inflammation diseases / Mini Rev Med Chem 14 (5) : 444 ~ 452
[학술지(정기간행물)] - Bordignon B / 2013 / Ascorbic acid derivatives as a new class of antiproliferative molecules / Cancer Lett 338 (2) : 317 ~ 327
[학술지(정기간행물)] - Kawada H / 2013 / High concentrations of L-ascorbic acid specifically inhibit the growth of human leukemic cells via downregulation of HIF-1alpha transcription / PLoS One 8 (4) : e62717 ~
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