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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.8
- 수록면
- 1,203 - 1,213 (11page)
- DOI
- 10.13000/JFMSE.2017.29.4.1203
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초록· 키워드
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The mud loach, Misgurnus mizolepis, are the important freshwater fish species for culture in Korea. Recently, mass mortality occurred among imported mud loach from China.
Clinical signs of diseased fish showed hemorrhage of operculum and mouth, abrasion of mouth and abnormal swimming. Internally, pale liver with hemorrhage, enlarged kidney and spleen were observed. And some of affected fish showed severe enteritis of the intestine.
Pure bacterial isolates were obtained with round, slightly convex and reddish-brown colonies on TSA medium after 48h incubation at 27℃. And the isolates could produce H₂S on peptone iron agar (PIA, BD). Using the API system, the isolates were identified as Shewanella putrefaciens (99.9%, 99.0%). The isolates grew well at 1 to 3% NaCl and its optimum concentration was 3%. Optimal pH range for growth was pH 6~8. And LD50 of SP-2 and L-2 calculated from the pathogenicity test were 1.7 × 10<SUP>7</SUP> cfu/fish and 1.2 × 10<SUP>7</SUP> cfu/fish, respectively. This is the first report on S. puterefaciens infection in freshwater fish in Korea.
Clinical signs of diseased fish showed hemorrhage of operculum and mouth, abrasion of mouth and abnormal swimming. Internally, pale liver with hemorrhage, enlarged kidney and spleen were observed. And some of affected fish showed severe enteritis of the intestine.
Pure bacterial isolates were obtained with round, slightly convex and reddish-brown colonies on TSA medium after 48h incubation at 27℃. And the isolates could produce H₂S on peptone iron agar (PIA, BD). Using the API system, the isolates were identified as Shewanella putrefaciens (99.9%, 99.0%). The isolates grew well at 1 to 3% NaCl and its optimum concentration was 3%. Optimal pH range for growth was pH 6~8. And LD50 of SP-2 and L-2 calculated from the pathogenicity test were 1.7 × 10<SUP>7</SUP> cfu/fish and 1.2 × 10<SUP>7</SUP> cfu/fish, respectively. This is the first report on S. puterefaciens infection in freshwater fish in Korea.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과
Ⅳ. 고찰
References
참고문헌 (26)
참고문헌 신청
Brink A. J van Straten A. and van Rensburg A. J. (1995). Shewanella (Pseudomonas) putrefaciens bacteremia. Clin. Infect. Dis., 20: 1327~1332.
Chen, Y. S. Liu, Y. C. Yen, M. Y. Wang, J. H. Wann, S. R. and Cheng, D. L.(1997). Skin and soft-tissue manifestations of Shewanella putrefaciens infection. Clin. Infect. Dis., 25: 225~229.
Dalgaard, P.(1995). Qualitatvie and quantitative characterization of spoilage bacteria from packed fish. Int. J. Food Microbiol., 26: 319~333.
Feldhusen, F.(2000). The role of seafood in bacterial foodborne diseases. Microbes. Infect. 2: 1651~1660.
Holt, H. M. Søgaard, P. and Gahrn-Hansen, B.(1997). Ear infections with Shewanella alga. A bacteriologic, clinical and epidemiologic study of 67 cases. Clin. Microbiol. Infect., 3: 329~334.
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