Molecular Cloning and Characterization of a P38-Like Mitogen-Activated Protein Kinase from Echinococcus granulosus

Guodong Lü; Jing Li; Chuanshan Zhang; Liang Li; Xiaojuan Bi; Chaowang Li; Jinliang Fan; Xiaomei Lu; Dominique A. Vuitton; Hao Wen; Renyong Lin

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저자정보: Guodong Lü (The First Affiliated Hospital of Xinjiang Medical University) Jing Li (The First Affiliated Hospital of Xinjiang Medical University) Chuanshan Zhang (The First Affiliated Hospital of Xinjiang Medical University) Liang Li (The First Affiliated Hospital of Xinjiang Medical University) Xiaojuan Bi (The First Affiliated Hospital of Xinjiang Medical University) Chaowang Li (The First Affiliated Hospital of Xinjiang Medical University) Jinliang Fan (The First Affiliated Hospital of Xinjiang Medical University) Xiaomei Lu (The First Affiliated Hospital of Xinjiang Medical University) Dominique A. Vuitton (University of Franche-Comté (EA 3181) and University Hospital) Hao Wen (The First Affiliated Hospital of Xinjiang Medical University) Renyong Lin (The First Affiliated Hospital of Xinjiang Medical University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.6

발행연도: 2016.12

수록면: 759 - 769 (11page)

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Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus granulosus. We identified a 1,107 bp cDNA encoding a 368 amino acid MAPK protein (Egp38) in E. granulosus. Egp38 exhibits 2 distinguishing features of p38-like kinases: a highly conserved T-X-Y motif and an activation loop segment. Structural homology modeling indicated a conserved structure among Egp38, EmMPK2, and H. sapiens p38α, implying a common binding mechanism for the ligand domain and downstream signal transduction processing similar to that described for p38α. Egp38 and its phosphorylated form are expressed in the E. granulosus larval stages vesicle and protoscolices during intermediate host infection of an intermediate host. Treatment of in vitro cultivated protoscolices with the p38-MAPK inhibitor ML3403 effectively suppressed Egp38 activity and led to significant protoscolices death within 5 days. Treatment of in vitro-cultivated protoscolices with TGF-β1 effectively induced Egp38 phosphorylation. In summary, the MAPK, Egp38, was identified in E. granulosus, as an anti-CE drug target and participates in the interplay between the host and E. granulosus via human TGF-β1.

#Echinococcus granulosus #cystic echinococcosis #protoscolex #p38 MAP kinase

참고문헌 (39)

참고문헌 신청

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