A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis

Tao Yang; Sibo Wang; Xuyong Zhang; Jie Xia; Jun Guo; Jixue Hou; Hongwei Zhang; Xueling Chen; Xiangwei Wu

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자료유형: 학술저널

저자정보: Tao Yang (Shihezi University School of Medicine) Sibo Wang (Shihezi University School of Medicine) Xuyong Zhang (Shihezi University School of Medicine) Jie Xia (Shihezi University School of Medicine) Jun Guo (Shihezi University School of Medicine) Jixue Hou (Shihezi University School of Medicine) Hongwei Zhang (Shihezi University School of Medicine) Xueling Chen (Shihezi University School of Medicine) Xiangwei Wu (Shihezi University School of Medicine)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.2

발행연도: 2016.4

수록면: 225 - 231 (7page)

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Human and animal alveolar echinococcosis (AE) are important helminth infections endemic in wide areas of the Northern hemisphere. Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite. Here, we generated a kind of fluorescent protoscolices in vivo imaging model and utilized this model to assess the activity against E. multilocularis protoscolices of metformin (Met). Results indicated that JC-1 tagged E. multilocularis can be reliably and confidently used to monitor protoscolices in vitro and in vivo. The availability of this transient in vivo fluorescent imaging of E. multilocularis protoscolices constitutes an important step toward the long term bio-imaging research of the AE-infected mouse models. In addition, this will be of great interest for further research on infection strategies and development of drugs and vaccines against E. multilocularis and other cestodes.

#Echinococcus multilocularis #fluorescent imaging #in vivo #animal model

참고문헌 (26)

참고문헌 신청

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