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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.8
- 수록면
- 461 - 469 (9page)
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Giardia lamblia is a protozoan that causes diarrheal diseases in humans. Cytoskeletal structures of Giardia trophozoites must be finely reorganized during cell division. To identify Giardia proteins which interact with microtubules (MTs), Giardia lysates were incubated with in vitro-polymerized MTs and then precipitated by ultracentifugation. A hypothetical protein (GL50803_8405) was identified in the precipitated fraction with polymerized MTs and was named GlMBP1 (G. lamblia microtubule-binding protein 1). Interaction of GlMBP1 with MTs was confirmed by MT binding assays using recombinant GlMBP1 (rGlMBP1). In vivo expression of GlMBP1 was shown by a real-time PCR and western blot analysis using anti-rGlMBP1 antibodies. Transgenic G. lamblia trophozoites were constructed by integrating a chimeric gene encoding hemagglutinin (HA)-tagged GlMBP1 into a Giardia chromosome. Immunofluorescence assays of this transgenic G. lamblia, using anti-HA antibodies, revealed that GlMBP1 mainly localized at the basal bodies, axonemes, and median bodies of G. lamblia trophozoites. This result indicates that GlMBP1 is a component of the G. lamblia cytoskeleton.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - Elmendorf HG / 2003 / The cytoskeleton of Giardia lamblia / Int J Parasitol 33 : 3 ~ 28
[학술지(정기간행물)] - Desai A / 1997 / Microtubule polymerization dynamics / Annu Rev Cell Dev Biol 13 : 83 ~ 117
[학술지(정기간행물)] - Akhmanova A / 2008 / Tracking the ends: a dynamic protein network controls the fate of microtubule tips / Nat Rev Mol Cell Biol 9 : 309 ~ 322
[학술지(정기간행물)] - Dawson SC / 2007 / Kinesin-13 regulates flagellar, interphase, mitotic microtubule dynamics in Giardia intestinalis / Eukaryot Cell 6 : 2354 ~ 2364
[학술지(정기간행물)] - Kim J / 2014 / Characterization of microtubule-binding and dimerization activity of Giardia lamblia end-binding 1 protein / PLoS One 9 : e97850 ~
[학술지(정기간행물)] - Desai A / 1997 / Microtubule polymerization dynamics / Annu Rev Cell Dev Biol 13 : 83 ~ 117
[학술지(정기간행물)] - Akhmanova A / 2008 / Tracking the ends: a dynamic protein network controls the fate of microtubule tips / Nat Rev Mol Cell Biol 9 : 309 ~ 322
[학술지(정기간행물)] - Dawson SC / 2007 / Kinesin-13 regulates flagellar, interphase, mitotic microtubule dynamics in Giardia intestinalis / Eukaryot Cell 6 : 2354 ~ 2364
[학술지(정기간행물)] - Kim J / 2014 / Characterization of microtubule-binding and dimerization activity of Giardia lamblia end-binding 1 protein / PLoS One 9 : e97850 ~
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UCI(KEPA) : I410-ECN-0101-2017-513-001124571