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Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea
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Type
Academic journal
Author
Ji-Hun Shin (Seoul National University College of Medicine) Sang-Eun Lee (Korea Center for Disease Control and Prevention) Tong Soo Kim (Inha University School of Medicine) Da-Won Ma (Korea Center for Disease Control and Prevention) Jong-Yil Chai (Seoul National University College of Medicine)
Journal
The Korean Society for Parasitology Parasites, Hosts and Diseases Vol.54 No.5 KCI Accredited Journals SCIE
Published
2016.10
Pages
631 - 636 (6page)

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Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler's Diarrhea
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This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively. The multiplex-touchdown PCR protocol using a primer mixture simultaneously detected protozoa in human stools, and the amplified gene was detected in >1×10³ oocysts for C. parvum, >1×10⁴ cysts for G. lamblia, and >1 copy of the 18S rRNA gene for C. cayetanensis. Taken together, our protocol convincingly demonstrated the ability to simultaneously detect C. parvum, G. lamblia, and C. cayetanenesis in stool samples.
#Cryptosporidium parvum #Giardia lamblia #Cyclospora cayetanensis #multiplex PCR #touchdown PCR #stool sample

Contents

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

References (30)

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[학술지(정기간행물)] - Diemert DJ / 2006 / Prevention and self-treatment of traveler's diarrhea / Clin Microbiol Rev 19 : 583 ~ 594 google schola [학술지(정기간행물)] - DuPont HL / 2006 / Travellers’ diarrhoea : contemporary approaches to therapy and prevention / Drugs 66 : 303 ~ 314 google schola [학술지(정기간행물)] - Wood AJ / 1993 / Prevention and treatment of traveler's diarrhea / New Engl J Med 328 : 1821 ~ 1827 google schola [단행본] - Bradley AC / 2016 / CDC Health Information for International Travel 2016 / Oxford University Press google schola [학술지(정기간행물)] - Freedman DO / 2006 / Spectrum of disease and relation to place of exposure among ill returned travelers / New Engl J Med 354 : 119 ~ 130 google schola

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