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학술저널
저자정보
Flavia Thamiris Figueiredo Pacheco (Federal University of Bahia) Silvia Souza de Carvalho (Federal University of Bahia) Samara Alves Santos (Federal University of Bahia) Gisele Maria Trindade das Chagas (Federal University of Bahia) Mariana Conceicao Santos (Federal University of Bahia) Jessica Gleide Souza Santos (Federal University of Bahia) Hugo da Costa-Ribeiro Junior (Federal University of Bahia) Tereza Cristina Medrado Ribeiro (Federal University of Bahia) Angela Peixoto de Mattos (Federal University of Bahia) Maria Aparecida Gomes (Federal University of Minas Gerais) Neci Matos Soares (Federal University of Bahia) Marcia Cristina Aquino Teixeira (Federal University of Bahia)
저널정보
대한진단검사의학회 Annals of Laboratory Medicine Annals of Laboratory Medicine 제40권 제5호
발행연도
2020.1
수록면
382 - 389 (8page)

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Background: Giardia duodenalis is conventionally diagnosed in fecal samples using parasitological methods. However, sensitivity is poor when only a single sample is analyzed, due to intermittent excretion of cysts in feces. Alternatively, the serum antibodies to G. duodenalis can be used for parasite diagnosis and epidemiological studies to determine previous exposure. We compared the rate of G. duodenalis infection between serum anti-Giardia IgG and IgA antibodies and fecal examination in Brazilian children. Methods: Fecal and serum samples were tested from 287 children at a clinical laboratory and from 187 children at daycare centers. Fecal samples were processed using conventional parasitological methods and coproantigen detection for Giardia diagnosis. Serum samples were tested using an in-house ELISA for detection of anti-Giardia IgG and IgA. Results: G. duodenalis was found in 8.2% (N=39) of the 474 children analyzed. The sensitivity and specificity of ELISA were 80.0% and 90.0% for IgG and 80.0% and 83.3% for IgA, respectively. The total positivity rate of anti-Giardia IgG and IgA in the sera was 13.9% (N=66) and 23.6% (N=112). The agreement between the positivity of specific antibodies and the detection of G. duodenalis in feces was moderate for ELISA-IgG, kappa index (95% CI)=0.543 (0.422?0.664), and mild for ELISA-IgA, kappa index (95% CI)=0.283 (0.162?0.404). Among the children infected with other enteroparasites, 11.6% (N=10) and 24.4% (N=21) showed reactivity to anti-Giardia IgG and to IgA, respectively. This cross-reactivity was more frequent in samples from children infected with Endolimax nana and Entamoeba coli. Conclusions: The higher frequency of specific antibody reactivity compared with G. duodenalis diagnosis in feces could reflect continuous exposure of children to G. duodenalis infection, resulting in long-lasting immunological memory and/or cross-reactivity with other intestinal amoebas.

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