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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 419 - 427 (9page)
이용수
초록· 키워드
오류제보하기
This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes of traveler’s diarrhea. Three target genes were specifically and simultaneously detected by the TaqMan probe method for multiple parasitic infection cases, including Cryptosporidium oocyst wall protein for C. parvum, glutamate dehydrogenase for G. lamblia, and internal transcribed spacer 1 for C. cayetanensis. Gene product 21 for bacteriophage T4 was used as an internal control DNA target for monitoring human stool DNA amplification. TaqMan probes were prepared using 4 fluorescent dyes, FAM™, HEX™, Cy5™, and CAL Fluor Red<SUP>®</SUP> 610 on C. parvum, G. lamblia, C. cayetanensis, and bacteriophage T4, respectively. We developed a novel primer-probe set for each parasite, a primer-probe cocktail (a mixture of primers and probes for the parasites and the internal control) for multiplex real-time PCR analysis, and a protocol for this detection method. Multiplex real-time PCR with the primer-probe cocktail successfully and specifically detected the target genes of C. parvum, G. lamblia, and C. cayetanensis in the mixed spiked human stool sample. The limit of detection for our assay was 2×10 copies for C. parvum and for C. cayetanensis, while it was 2×103 copies for G. lamblia. We propose that the multiplex real-time PCR detection method developed here is a useful method for simultaneously diagnosing the most common causative protozoa in traveler’s diarrhea.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Diemert DJ / 2006 / Prevention and self-treatment of traveler's diarrhea / Clin Microbiol Rev 19 : 583 ~ 594
[학술지(정기간행물)] - DuPont HL / 2006 / Travellers’ diarrhoea : contemporary approaches to therapy and prevention / Drugs 66 : 303 ~ 314
[학술지(정기간행물)] - de la Cabada Bauche J / 2011 / New developments in traveler's diarrhea / Gastroenterol Hepatol 7 : 88 ~ 95
[학술지(정기간행물)] - DuPont HL / 2009 / Systematic review: the epidemiology and clinical features of travellers’ diarrhoea / Aliment Pharmacol Ther 30 : 187 ~ 196
[학술지(정기간행물)] - Baldursson S / 2011 / Waterborne transmission of protozoan parasites : review of worldwide outbreaks-an update 2004-2010 / Water Res 45 : 6603 ~ 6614
[학술지(정기간행물)] - DuPont HL / 2006 / Travellers’ diarrhoea : contemporary approaches to therapy and prevention / Drugs 66 : 303 ~ 314
[학술지(정기간행물)] - de la Cabada Bauche J / 2011 / New developments in traveler's diarrhea / Gastroenterol Hepatol 7 : 88 ~ 95
[학술지(정기간행물)] - DuPont HL / 2009 / Systematic review: the epidemiology and clinical features of travellers’ diarrhoea / Aliment Pharmacol Ther 30 : 187 ~ 196
[학술지(정기간행물)] - Baldursson S / 2011 / Waterborne transmission of protozoan parasites : review of worldwide outbreaks-an update 2004-2010 / Water Res 45 : 6603 ~ 6614
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