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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.43 No.2
- 발행연도
- 2016.6
- 수록면
- 205 - 214 (10page)
이용수
초록· 키워드
오류제보하기
A drought-tolerant transgenic rice (Agb0103) was developed using a pepper methionine sulfoxide reductase (CaMsrB2) under the control of rice Rab21 promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. Commercialization of genetically modified (GM) crops will require the evaluation of risks associated with the release of GM crops. With the potential problems associated to GM crops safety testing, the investigation of their effects on non-target organisms is necessary for environmental risk research. This study was carried out to assess acute toxicity of a GM crop using the water flea (Daphnia magna) for non-target organism risk evaluation. The effect of acute toxicity on Daphnia magna of Agb0103 rice and a non-GM rice, Ilmibyeo, were investigated at different concentrations (0, 625, 1,250, 2,500, 5,000, and 10,000 ㎎/L). The Agb0103 rice used for the test was confirmed to express the CaMsrB2/PAT gene by the PCR and ELISA. Daphnia magna feeding tests showed no significant differences in cumulative immobility or abnormal response with either Agb0103 rice or non-GM rice. The 48hr-EC<SUB>50</SUB> values showed no difference between Agb0103 rice (2243 ㎎/L) and non-GM rice (2694 ㎎/L). These results suggest that there is no significant difference in toxicity to Daphnia magna between Agb0103 rice and its non-GM counterpart.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (19)
참고문헌 신청
[학술지(정기간행물)] - Ha SH / 2010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / Plant Biotechnology 8 : 928 ~ 938
[보고서] - James C / 2014 / The global status of commercialized biotech/GM crops: 2014
[학술지(정기간행물)] - Jung S / 2004 / Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance / Plant Cell Environment 27 : 1436 ~ 1446
[학술지(정기간행물)] - Kim JS / 2014 / A pepper Msrb2 gene confers drought tolerance in rice through the protection of chloroplast targeted genes / Plos one 9 : e90588 ~
[학술지(정기간행물)] - Kim HJ / 2010 / Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea / Journal of Agriculture & Food Chemistry 58 : 10906 ~ 10910
[보고서] - James C / 2014 / The global status of commercialized biotech/GM crops: 2014
[학술지(정기간행물)] - Jung S / 2004 / Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance / Plant Cell Environment 27 : 1436 ~ 1446
[학술지(정기간행물)] - Kim JS / 2014 / A pepper Msrb2 gene confers drought tolerance in rice through the protection of chloroplast targeted genes / Plos one 9 : e90588 ~
[학술지(정기간행물)] - Kim HJ / 2010 / Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea / Journal of Agriculture & Food Chemistry 58 : 10906 ~ 10910
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UCI(KEPA) : I410-ECN-0101-2017-520-000856891