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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.2
- 수록면
- 59 - 68 (10page)
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초록· 키워드
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For comparison of tetracycline-resistant (Tc<SUP>R</SUP>) genes, we obtained 21 and 14 Tc<SUP>R</SUP> Staphylococcus spp. from marine environment and human patient, respectively. Although all isolates from human were identified as Staphylococcus aureus, higher proportion of Tc<SUP>R</SUP> isolates (12 out of 14) from human were utilizing tet(M) gene compared to that of Tc<SUP>R</SUP> isolates (6 out of 21) from marine environment. Additionally, collaborated utilization of tet(M) and erm(A) in Tc<SUP>R</SUP>-Em<SUP>R</SUP> S. aureus in human patient, but not in Tc<SUP>R</SUP> Staphylococcus spp. isolates from marine environment was also characterized. Based on the nucleotide sequence of transposon related to Tc<SUP>R</SUP> gene, we confirmed the origin of tet(M) gene in Tc<SUP>R</SUP> Staphylococci isolated from marine environments and human are derived from Tn916/1545-like and Tn5801 transposon, respectively. It is the first report showing the presence of Tn5801 in all Tc<SUP>R</SUP> S. aureus carrying tet(M) in human patient. Alignment of the fully sequenced tet(M) from marine environmental isolates was also agreed with the determined transposons by showing the genomic mosaic structure composed with three genomic parts from Tn916/1545 and unknown transposons. Genetic characteristics of these tet(M) in environmental isolates were similar to each other but different from those in isolates from human showing only tet(M) from Tn916/5801 type. It may imply the presence of less dramatic communication of antibiotic resistant genes between Staphylococci isolated from marine environment and human.
목차
Abstract
Ⅰ. 서론
Ⅱ. 조사방법 및 내용
Ⅲ. 결과
Ⅳ. 고찰
References
참고문헌 (20)
참고문헌 신청
CLSI. (2012). Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. Document M100-S22. CLSI, Wayne PA Vol. 32
Courvalin, P, Carlier, C.(1987). Tn1545: a conjugative shuttle transposon. Mol. Gen. Genet 206, 259~264.
De Vries, L. E. Christensen, H. Skov, R. L. Aarestrup, F. M. Agersø, Y.(2009). Diversity of the tetracycline resistance gene tet(M) and identification of Tn916- and Tn5801-like (Tn6014) transposons in Staphylococcus aureus from humans and animals. J Antimicrob Chemother 64(3), 490~500.
Eady, E. A. Ross, J. I. Tipper, J. L. Walters, C. E. Cove, J. H. Noble, W. C.(1993). Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. J Antimicrob Chemother 31(2), 211~217.
Fernandez-Astorga, A. Muela, A. Cisterna, R. Iriberri, J. Barcina, I.(1992). Biotic and abiotic factors affecting plasmid transfer in Escherichia coli strains. Appl Environ Microbiol 58, 392~398.
Courvalin, P, Carlier, C.(1987). Tn1545: a conjugative shuttle transposon. Mol. Gen. Genet 206, 259~264.
De Vries, L. E. Christensen, H. Skov, R. L. Aarestrup, F. M. Agersø, Y.(2009). Diversity of the tetracycline resistance gene tet(M) and identification of Tn916- and Tn5801-like (Tn6014) transposons in Staphylococcus aureus from humans and animals. J Antimicrob Chemother 64(3), 490~500.
Eady, E. A. Ross, J. I. Tipper, J. L. Walters, C. E. Cove, J. H. Noble, W. C.(1993). Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. J Antimicrob Chemother 31(2), 211~217.
Fernandez-Astorga, A. Muela, A. Cisterna, R. Iriberri, J. Barcina, I.(1992). Biotic and abiotic factors affecting plasmid transfer in Escherichia coli strains. Appl Environ Microbiol 58, 392~398.
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UCI(KEPA) : I410-ECN-0101-2016-374-002373091