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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 834 - 846 (13page)
- DOI
- 10.13000/JFMSE.2017.29.3.834
이용수
이 논문의 연구 히스토리 (3)
2018
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초록· 키워드
오류제보하기
Aquaculture practices to ensure greater production, such as high density breeding and excessive feeding, are become stressors that raise the prevalence of diseases. Accordingly, increasingly large volumes of antibiotics are used more frequently each year. Long term use antibiotics can generate resistant bacteria, which interrupt treatments and cause a potential transfer to human bodies. Thus, antibiotic resistance is of importance in public health. Tetracycline (Tc) is one of the typical medicines used in the aquaculture drugs, which has a wide range of application including gram-positive and gram-negative bacteria. In the examination of 153 strains isolated from olive flounder (Paralichthys olivaceus) farms located in Jeju in 2016, it turned out that a total of 84 strains were resistant to Tc or oxytetracycline (OTC). The extent to which the strains are resistant to Tc and OTC was confirmed through MIC test, mostly within the range of 25 to 100 ㎍/㎖. Twelve different types of tet genes were detected using single and multiplex PCR in the 84 Tc-resistant strains. The PCR was used to find tet(K), tet(M), tet(O), and tet(S), which are known to exist primarily in gram–positive strains. According to the results, tet(S) is the most dominant gene in 49 strains of Streptococcus parauberis, accounting for 63.2%. And there were two strains that have two different types of resistant genes. The multiplex PCR was used to detect tet(A), tet(B), tet(C), tet(D), tet(E), and tet(G), which are commonly found in gram-negative strains. Each of tet(B), tet(D), and tet(B)&(M) was found in a strain presumed to be Vibrio sp., and only tet(D) was found in 10 Edwardsiella tarda strains.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과
Ⅳ. 고찰
References
참고문헌 (23)
참고문헌 신청
Chopra, I. and Roberts, M. C.(2001). Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance. Microbiology and Molecular Biology Reviews 65(2), 232~260.
Heo, J. H. Jung, M. H. Cho, M. H. Kim, G. H. Lee, K. C. Kim, J. H. and Jung, T. S.(2002). The study on fish diseases with reference to bacterial susceptibility to antibiotics in the southern area of Kyeognam. Journal of Veterinary Clinics 19(1), 19~22
Jee, B. Y. Shin, K. W. Lee, D. W. Kim, Y. J. and Lee, M. K.(2014). Monitoring of the mortalities and medications in the inland farms of olive flounder, Paralichthys olivaceus, in South Korea. Journal of Fish Pathology 27(1), 77~83.
Jun, L. J.(2003). Characterization of tet genes in fish pathogenic bacteria from Korea. Master's Thesis, Department of Fish Pathology, Pukyong National University, Busan, South Korea.
Jun, L. J.(2010). Characterization of antibiotic resistant genes carried by fish pathogens in Korea. Ph.D. Thesis, Department of Fish Pathology, Pukyong National University, Busan, South Korea.
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UCI(KEPA) : I410-ECN-0101-2018-454-000907803