지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 241 - 247 (7page)
이용수
초록· 키워드
오류제보하기
Lactate and ketone bodies are considered biological markers for ketosis and several inherited metabolic disorders. In the current study, the specific ratios of lactate and ketone bodies as analytical tools for differential diagnosis of various lactic acidosis were devised. The study included a protein precipitation step following tert-butyldimethylsilyl derivatisation. Total run time was approximately 30 min including sample preparation and GS/MS analysis. The limits of detection were below 0.1 pg/mL over the targeted 4 analytes. The calibration curve was linear over the concentration range of 0.001~250 μg/mL for pyruvate, beta-hydroxybutyrate, and acetoacetate (R² > 0.99). Inter-day accuracy and precision were 87.7~94.8% with RSD of 2.5~5.7% at 2 levels. Absolute recoveries (%) of target analytes were 87.0~98.4%. The method was validated for the quantification of lactate and ketone bodies for differentiation of lactic acidosis.
목차
INTRODUCTION
MATERIAL AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Amathieu R / 2014 / Serum 1H-NMR metabolomic fingerprints of acute-onchronic liver failure in intensive care unit patients with alcoholic cirrhosis / PLoS One 19 : e89230 ~
[학술지(정기간행물)] - Bhattacharya M / 1995 / Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli / Anal Biochem 232 : 98 ~ 106
[학술지(정기간행물)] - Chaves Das Heves / 1987 / Capillary gas chromatography of amino acids, including asparagine and glutamine: sensitive gas chromatographic-mass spectrometric and selected ion monitoring gas chromatographic-mass spectrometric detection of the N,O(S)-tert-butyldimethylsilyl derivatives / J Chromatogr 392 : 249 ~ 258
[학술지(정기간행물)] - Constantin-Teodosiu D / 1991 / A sensitive radioisotopic assay of pyruvate dehydrogenase complex in human muscle tissue / Anal Biochem 198 : 347 ~ 351
[학술지(정기간행물)] - Dahl SR / 2012 / Determination of gamma-hydroxy butyrate (GHB), beta-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and gamma-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS / J Chromatogr B 885 : 37 ~ 42
[학술지(정기간행물)] - Bhattacharya M / 1995 / Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli / Anal Biochem 232 : 98 ~ 106
[학술지(정기간행물)] - Chaves Das Heves / 1987 / Capillary gas chromatography of amino acids, including asparagine and glutamine: sensitive gas chromatographic-mass spectrometric and selected ion monitoring gas chromatographic-mass spectrometric detection of the N,O(S)-tert-butyldimethylsilyl derivatives / J Chromatogr 392 : 249 ~ 258
[학술지(정기간행물)] - Constantin-Teodosiu D / 1991 / A sensitive radioisotopic assay of pyruvate dehydrogenase complex in human muscle tissue / Anal Biochem 198 : 347 ~ 351
[학술지(정기간행물)] - Dahl SR / 2012 / Determination of gamma-hydroxy butyrate (GHB), beta-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and gamma-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS / J Chromatogr B 885 : 37 ~ 42
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UCI(KEPA) : I410-ECN-0101-2016-510-002322041