지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.88 No.1
- 발행연도
- 2014.12
- 수록면
- 1 - 7 (7page)
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초록· 키워드
오류제보하기
Purpose: The aim of this study was to establish an in vitro method to purify human multipotent adult progenitor cells (hMAPCs) and assess their possible differentiation into hepatocytes by coculture with human hepatocyte line L02.
Methods: hMAPCs were isolated by magnetic activated cell sorting (MACS) depletion selection using CD45 and GlyA microbeads. After indirect or direct coculture of hMAPCs and human hepatocyte line L02, the expression of albumin (ALB), alpha-fetoprotein (AFP), cytokeratin (CK) 18, and CK19 by hMAPCs was detected by immunocytochemistry.
Results: With the MACS method, (5?10) × 10⁴/mL hMAPCs could be separated from 1 × 10?/mL bone marrow mononuclear cells. The purity of CD45-/GlyA- cells separated from bone marrow adherent cells was more than 98%, as determined by flow cytometry. In the coculture without cell-to-cell contact, hMAPCs expressed high AFP on day 1, and then tapered daily to low expression on day 7; ALB expression reached its peak on day 5, and remained high on day 7; CK18 was initially expressed on day 5 and was higher on day 7; CK19 was negative in all assays. In the coculture with cell-to-cell contact, ALB and CK18 were expressed by most cells while AFP appeared in only a few on day 5.
Conclusion: hMAPCs were induced to differentiate into mature hepatocyte-like cells by coculture with a hepatocyte cell line, either with or without cell-to-cell contact, but the former seemed more effective.
Methods: hMAPCs were isolated by magnetic activated cell sorting (MACS) depletion selection using CD45 and GlyA microbeads. After indirect or direct coculture of hMAPCs and human hepatocyte line L02, the expression of albumin (ALB), alpha-fetoprotein (AFP), cytokeratin (CK) 18, and CK19 by hMAPCs was detected by immunocytochemistry.
Results: With the MACS method, (5?10) × 10⁴/mL hMAPCs could be separated from 1 × 10?/mL bone marrow mononuclear cells. The purity of CD45-/GlyA- cells separated from bone marrow adherent cells was more than 98%, as determined by flow cytometry. In the coculture without cell-to-cell contact, hMAPCs expressed high AFP on day 1, and then tapered daily to low expression on day 7; ALB expression reached its peak on day 5, and remained high on day 7; CK18 was initially expressed on day 5 and was higher on day 7; CK19 was negative in all assays. In the coculture with cell-to-cell contact, ALB and CK18 were expressed by most cells while AFP appeared in only a few on day 5.
Conclusion: hMAPCs were induced to differentiate into mature hepatocyte-like cells by coculture with a hepatocyte cell line, either with or without cell-to-cell contact, but the former seemed more effective.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (18)
참고문헌 신청
1. [학술지(정기간행물)] - Li M. / 2013 / Bone-marrow-derived mesenchymal stem cells for organ repair / Stem Cells Int 2013 : 132642 ~
2. [학술지(정기간행물)] - Prasajak P. / 2013 / Developing a new two-step protocol to generate functional hepatocytes from Wharton's Jellyderived mesenchymal stem cells under hypoxic condition / Stem Cells Int 2013 : 762196 ~
3. [학술지(정기간행물)] - Ayatollahi M. / 2011 / Hepatogenic differentiation of mesenchymal stem cells induced by insulin like growth factor-I / World J Stem Cells 3 : 113 ~ 121
4. [학술지(정기간행물)] - Chen XW. / 2012 / Therapeutic effect of transplanting magnetically labeled bone marrow stromal stem cells in a liver injury rat model with 70%-hepatectomy / Med Sci Monit 18 : BR375 ~ BR382
5. [학술지(정기간행물)] - Jiang Y. / 2003 / Neuroectodermal differentiation from mouse multipotent adult progenitor cells / Proc Natl Acad Sci U S A 100 (Suppl 1) : 11854 ~ 11860
2. [학술지(정기간행물)] - Prasajak P. / 2013 / Developing a new two-step protocol to generate functional hepatocytes from Wharton's Jellyderived mesenchymal stem cells under hypoxic condition / Stem Cells Int 2013 : 762196 ~
3. [학술지(정기간행물)] - Ayatollahi M. / 2011 / Hepatogenic differentiation of mesenchymal stem cells induced by insulin like growth factor-I / World J Stem Cells 3 : 113 ~ 121
4. [학술지(정기간행물)] - Chen XW. / 2012 / Therapeutic effect of transplanting magnetically labeled bone marrow stromal stem cells in a liver injury rat model with 70%-hepatectomy / Med Sci Monit 18 : BR375 ~ BR382
5. [학술지(정기간행물)] - Jiang Y. / 2003 / Neuroectodermal differentiation from mouse multipotent adult progenitor cells / Proc Natl Acad Sci U S A 100 (Suppl 1) : 11854 ~ 11860
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UCI(KEPA) : I410-ECN-0101-2016-514-001071514