지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1996.12
- 수록면
- 163 - 171 (9page)
이용수
초록· 키워드
오류제보하기
The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gPSO. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protectivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Pseudorabies Virus 의 Major Capsid Protein 유전자의 클론닝과 Baculovirus Vector System에 의한 발현
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1996 .12
-
Expression of Neutralizing Proteins of Pseudorabies Virus using Recombinant Baculovirus
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1992 .06
-
Swine Influenza Virus Recombinant HA Protein Expressed in the Baculovirus System Elicited Protective Immunity in Mice
Laboratory Animal Research
2004 .06
-
곤충세포에서 사람파필로마바이러스 16형 L1 재조합 단백질의 생산 및 바이러스 유사 입자분리
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1995 .12
-
A mutated recombinant subunit vaccine protects mice and guinea pigs against botulinum type A intoxication
한국실험동물학회 학술발표대회 논문집
2017 .02
-
Effect of recombinant baculovirus vector containing anti-cancer gene, p53
한국실험동물학회 학술발표대회 논문집
2019 .01
-
단크론성항체를 이용한 돼지가성광견병 진단법 개발에 관한 연구
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1987 .06
-
Expression of Polyhistidine-Containing Fusion Human HepG2 Type Glucose Transport Protein in Spodoptera Cells and Its Purification Using a Metal Affinity Chromatography
대한의생명과학회지
2010 .09
-
분만후 임상적으로 회복상태인 암돼지에서의 Pseudorabies Viruses의 방출
대한수의사회지
1981 .01
-
Recombinant DNA and Protein Vaccines for Foot-and-mouth Disease Induce Humoral and Cellular Immune Responses in Mice
Immune Network
2009 .12
-
꿩에서 분리된 Newcastle Disease Virus 내열성주 (CBP)의 Fusion(F) 유전자 클론닝과 염기서열 분석
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1998 .09
-
Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2011 .09
-
Newcastle Disease Virus의 F와 HN 유전자 재조합 DNA 제조 및 면역원성
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2006 .06
-
Production of Recombinant Anti-Cancer Vaccines in Plants
Biomolecules & Therapeutics(구 응용약물학회지)
2017 .01
-
Expression of recombinant baculoviral vectors in tumor cells and tissues
한국실험동물학회 학술발표대회 논문집
2018 .01
-
Evaluation of Protective Immunity of Peptide Vaccines Composed of a 15-mer N-terminal Matrix Protein 2 and a Helper T-Cell Epitope Derived from Influenza A Virus
Immune Network
2019 .08
-
Construction of a Novel Recombinant Baculovirus Producing Polyhedra with a Bacillus thuringiensis Cry1Ac Crystal Protein
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1999 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0