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학술저널
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대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY 大韓바이러스學會誌 제24권 제2호
발행연도
1994.12
수록면
155 - 159 (5page)

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Approximately 40 separate adenoviruses have been isolated from man. Adenoviruses replicate in the nuclei of infected cells. They are 70 nm to 90 nm in diameter, and each capsid is composed of 252 capsomers. All members appear to have a eore composed of single molecule of DNA, and a common complement fixing structural virus antigen. Adenoviruses infection in man produce acute pharyngitis, trachcobronchitis, broncho- pneumonia or conjunctivitis. Several members of this group, including types commonly associated with respiratory disease in man, are capable of producing malignant tumors in young hamster and a few types have been shown to be oncogenic in young rat. Previous reports involving production and release of adenoviruses and its antigen have been carried out. The present report represents a continuation of previous study. To obtain evidence concerning the relationship between CF antigen and infectious virus, investigation of adenoviruses type 12 of this group wss undertaken. For practical consideration it was disirable to investigate of growth characteristics and its antigen induction in HeLa, FB and MK cells(permissive cells). Results were as follows. 1. CF antigen and infectious adenoviruses type 12 viruses were produced independently and the CF antigen was not a precusor to infectious adenoviruses type 12. 2. Intracellular CF antigen had eclipse period of 16-22 hrs and intracellular infectious virus was detected 16-23hrs after infection of HeLa, FB and MK cells. 3. HeLa and MK cells infected with adenoviruses type 12 were capable of continued antigen production despite early development of maximum cytopathogenic effect. 4. This study with adenovirus type 12 indicated this virus has similar growth characteristics in HeLa, KB and MK cells.

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