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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1977.6
- 수록면
- 7 - 18 (12page)
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Macrophage, scavenger cell against foreign materials, may play an important role cn hcst defense mechanism, maintenance of homeostasis function, and various immune resporses. However, it is not certain wheather or not those function of macrophages derived from patients who were treated with immuncsuppressive drugs may be kept well as in normal persens. To investigate the effect of immuncsuppressive drugs (prednisolone, cyclophcsphamide and vincristine sulfate) on mcuse peritoneal macrophages against Japanese encephalitis virus(JE virus) infecticn, each drug was dissolved and adjusted with physiological saline at the proper concentration(prednisolone 20mg/kg B.W./day, 200mg/kg B.W./day; cyclcsphamide 10mg/kg B.W./day, 100mg/kg B.W./day; vincristine sulfate 0.05mg/kg B.W. /day, 0.5mg/kg B.W./day). Then, were injected intraperitoneally with each drug solution for three corsecutive days. In 24 hours after last three groups of mice injection, peritoneal macrophages were isolated. And then macrophage layers were formed both the inner surface of Leightcn tube and on the coversligs of Leighten tube. Thereafter, JE virus was inoculated on macrophage monolayer ar.d cultured at 37 C. At apprcpriate time interval, macrophage cultvres were sempled. JE virus antigrs retained in the macrophage were detected by immuncfluorescence technique, and viral infectivity was measured by the plaque-forming methad on the monolayer of chick embryo fibroblasts. Peritoneal macrophages obtained from untreated nomal mice were used as the control. The results were summarized as follows: 1. On the cultures of normal peritoneal macrophage phagccytized Japarese cncephslitis virus, mezximal rate of macrophages retaining JE virus antigens was reachcd at 6 hours after virus adsorption. Thereafter, the rate of immunofluorescent cells was rapidly decreased, but low rate was still observed at 96 hours after virus inoculation. In contrast, on the cultures of peritoneal macrophages obtained from mice that were pretreated with immunosuppressive drug, such as prednisolone, cyclophosphamide and vincristine sulfate, the rate of macrophage retaining JE virus antigens was higher than that of untreated control macrophages. Duration necessary for reaching to the maximal rate was required approxinately 2-6 hours more than that of the control macrophages.2. Within 24 hours after the virus inoculation, virus infectivity was rapidly inactivated in cell-free medium, but inact!vation of virus infectivity in tbe macrophage cultures was more rapid than that of cell-free medium. However, virus infectivity recovered from macrophage cultures, even though low in titer, was continuously maintained throughout 96 hours. Recovery rate of the infectivity, which was observed from macrophage cultures from immunosuppressant pretreated mice, was always bigher than that of the untreated control mcrophage cultures.
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UCI(KEPA) : I410-ECN-0101-2016-475-001514725