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논문 기본 정보

자료유형
학술저널
저자정보
Yu Jihn Kwon (Gyeongin Regional Food and Drug Administration) So Young Chung (Gyeongin Regional Food and Drug Administration) Kyung Chul Cho (Gyeongin Regional Food and Drug Administration) ji Eun Park (Gyeongin Regional Food and Drug Administration) Eun Joo Koo (Gyeongin Regional Food and Drug Administration) Dong Hyuk Seo (Gyeongin Regional Food and Drug Administration) Eugene Kim (Gyeongin Regional Food and Drug Administration) Jehyun Whang (Gyeongin Regional Food and Drug Administration) Seong Soo Park (Gyeongin Regional Food and Drug Administration) Sun Ok Choi (Gyeongin Regional Food and Drug Administration) Chul Joo Lim (Gyeongin Regional Food and Drug Administration)
저널정보
한국분석과학회 분석과학 분석과학 제28권 제2호
발행연도
2015.4
수록면
117 - 124 (8page)

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초록· 키워드

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Genetically modified (GM) papaya line 55-1, which is resistant to PRSV infection, has been marketed globally. Prompt and sensitive protocols for specific detections are essential for the traceability of this line. Here, an event- and construct-specific real-time polymerase chain reaction (RT-PCR) method was established to detect 55-1. Qualitative detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% for the homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The method was applied in the qualitative detection of 55-1 in eight types of commercially processed papaya products. Additionally, papaya products were monitored to distinguish GM papaya using the P35S and T-nos RT-PCR detection methods. As expected, detection capacity was improved via modified sample preparation and the established RT-PCR detection method. Taking these results together, it can be suggested that a suitable method for the extraction and purification of DNA from processed papaya products was established for the detection of GM papaya.

목차

Abstract
1. Introduction
2. Materials and Methods
3. Results and Discussion
References

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UCI(KEPA) : I410-ECN-0101-2016-433-001460999