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자료유형
학술저널
저자정보
저널정보
한국사회체육학회 한국사회체육학회지 한국사회체육학회지 제16권
발행연도
2001.11
수록면
629 - 638 (10page)

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The purpose of this study was to evaluate of endurance exercises for 8 week on transformational change of the skeletal myosin heavy chain(MHC) isoforms at transcriptional and translational levels in the hindlimb musculature of Sprague-Dawley rats. All animals were divided into non-exercise control group endurance exercise group. In exercise groups animals received endurance treadmill training for 8 week. SDS-glycerol polyacrylamide gel electrophoresis(PAGE) to measure that of MHC protein. After finishing exercise the soleus, medial gastrocnemius(MGC) on the hind limb were excised, fixed quickly with liquid nitrogen to prevent destruction MHC protein. Myosin heavy chain protein was isolated from these muscles with extraction buffer and loaded on SDS - polyacrylamide gel containing 30% glycerol to separate several kinds of MHC isoforms. The results of this study were followed as: 1. After endurance 8 weeks there was significant reduction of body weight but significant increase of muscle weight and ratio of muscle weight to body weight in all the muscles weight, which meant these exercises induced muscular hypertrophy. 2. Comparing control group, endurance exercise group showed significant increase in type I MHC protein and significant decrease in type IIa MHC protein of the soleus muscle on the myosin SDS-glycerol PAGE study. 3. In the MGC, endurance exercise group resulted in significant increase for proportion of type I and significant decrease of that of type IIb. 4. Onthe myosin SDS-glycerol PAGE study. there was noted significant increase of percent ratio of type I in endurance exercise group. and decrement of type IIb MHC isoprotein proportion was measured in endurance exercise. From above results it could suggest that exercise induce expressional change of myosin heavy chain protein in the hindlimb musculature of rats.

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