지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Background & Objective : The aim of this study was to investigate the effect of P. radix on the inflammatory related gene expression in IL-1β-stimulated primary human gingival fibroblast using Whole Transcript Sense Target (WT-ST).
Method : Human gingival fibroblast was incubated with P. radix [100 or 200 ㎍/㎖], and IL-1β [1ng/㎖] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling Assay<SUP>®</SUP>.
Result : In the DEG results, 782 genes were up-regulated in the IL-1β-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-1β and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-1β-treated when compared to IL-1β-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-1β-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix 200㎍/㎖ plus IL-1β-treated group appear to be more involved in the metabolism-related pathways than in the 100㎍/㎖ plus IL-1β-treated group.
Conclusion & Discussion : By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.
Method : Human gingival fibroblast was incubated with P. radix [100 or 200 ㎍/㎖], and IL-1β [1ng/㎖] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling Assay<SUP>®</SUP>.
Result : In the DEG results, 782 genes were up-regulated in the IL-1β-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-1β and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-1β-treated when compared to IL-1β-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-1β-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix 200㎍/㎖ plus IL-1β-treated group appear to be more involved in the metabolism-related pathways than in the 100㎍/㎖ plus IL-1β-treated group.
Conclusion & Discussion : By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.
목차
서론
실험
결과
고찰
결론
감사의 글
참고문헌
참고문헌 (37)
참고문헌 신청
1. [학술지(정기간행물)] - Chang YC / 2003 / Induction of cyclooxygenase-2 mRNA and protein expression in human gingival fibroblasts stimulated with nicotine / J Periodont Res 38 : 496 ~ 501
2. [학술지(정기간행물)] - Chang YC / 2005 / The upregulation of heme oxygenase-1 expression in human gingival fibroblasts stimulated with nicotine / J Periodont Res 40 : 252 ~ 257
3. [학술지(정기간행물)] - Jordan HV / 1966 / In vitro methods for the study of plaque formation and carious lesions / Arch Oral Biol 11 : 793 ~ 802
4. [학술지(정기간행물)] - Theilade E / 1996 / Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation / J Periodontal Res 1 : 1 ~ 13
5. [학술지(정기간행물)] - Socransky SS / 1970 / Relationship of bacteria to the etiology of periodontal disease / J Dent Res 49 : 203 ~ 222
2. [학술지(정기간행물)] - Chang YC / 2005 / The upregulation of heme oxygenase-1 expression in human gingival fibroblasts stimulated with nicotine / J Periodont Res 40 : 252 ~ 257
3. [학술지(정기간행물)] - Jordan HV / 1966 / In vitro methods for the study of plaque formation and carious lesions / Arch Oral Biol 11 : 793 ~ 802
4. [학술지(정기간행물)] - Theilade E / 1996 / Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation / J Periodontal Res 1 : 1 ~ 13
5. [학술지(정기간행물)] - Socransky SS / 1970 / Relationship of bacteria to the etiology of periodontal disease / J Dent Res 49 : 203 ~ 222
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UCI(KEPA) : I410-ECN-0101-2014-519-000285435