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논문 기본 정보

자료유형
학술저널
저자정보
Seong-Hee Kim (Ministry of Agriculture and Forestry) Jin-Young Kim (Jeju Provincial Institute for Live Stock Promotion) Woong Han (ViroMed Co., Ltd.) Byeong Yeal Jung (Ministry of Agriculture and Forestry) Pham Duc Chuong (Thainguyen University of Agriculture and Forestry) Haejin Joo (Cheju National University) Hoa Van Ba (Cheju National University) Won-Geun Son (Cheju National University) Youngheun Jee (Cheju National University) Byoung-Su Yoon (Kyonggi University) Yong-Soon Lee (Seoul National University) Yoon-Kyu Lim (Cheju National University)
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 16. no 4
발행연도
2007.8
수록면
515 - 519 (5page)

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초록· 키워드

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Rapid immunochromatographic assay (ICA) kits were developed using flagella-specific monoclonal antibodies (MAbs) and rabbit polyclonal antibodies for screening Listeria spp. in food. The establishment of different formats, MAb 2B1 as capture antibody and MAb 7A3 or rabbit polyclonal antibodies as detector antibody, was compared. The 2 formats of the ICA kit were shown to have specific reactions with Listeria and no cross-reactivity with any of the non-Listeria including Escherichia coli O157:H7 and Salmonella enteritidis. The detection limits of the ICA kit using the combination of goldlabeled MAb 7A3 and MAb 2B1 showed 1×105 and 1×106 CFU/ 0.1 mL at 22 and 30oC, respectively. The other format of the ICA kit using the combination of gold-labeled rabbit polyclonal antibodies and MAb 2B1 showed 1×106 CFU/ 0.1 mL at 22oC but weak signal at 30 culture. The format utilizing MAb was more sensitive than the one using polyclonal antibodies for capture antibody. Samples contaminated with L. monocytogenes 4b culture (9-10, 5-6, and 1-2 CFU/mL) on pork and pasteurized milk were confirmed as positive results. Current data suggests that this ICA kit is a rapid, simple and effective tool to screen for Listeria spp. in food.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References

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UCI(KEPA) : I410-ECN-0101-2013-573-001023122