지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with Vλ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.
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참고문헌 (16)
참고문헌 신청
1. [학술지(정기간행물)] - Bird RE / 1988 / Single-chain antigen-binding proteins / Science 242 : 423 ~ 426
2. [학술지(정기간행물)] - Chapman HD / 1993 / Resistance to anticoccidial drugs in fowl / Parasitol Today 9 : 159 ~ 162
3. [학술지(정기간행물)] - Hayden MS / 1997 / Antibody engineering / Curr Opin Immunol 9 : 201 ~ 212
4. [학술지(정기간행물)] - Huston JS / 1991 / Protein engineering of singlechain Fv analogs and fusion proteins / Methods Enzymol 203 : 46 ~ 88
5. [단행본] - Kabat EA / 1991 / Sequences of proteins of immunological interest / NIH publication : 91 ~ 3242
2. [학술지(정기간행물)] - Chapman HD / 1993 / Resistance to anticoccidial drugs in fowl / Parasitol Today 9 : 159 ~ 162
3. [학술지(정기간행물)] - Hayden MS / 1997 / Antibody engineering / Curr Opin Immunol 9 : 201 ~ 212
4. [학술지(정기간행물)] - Huston JS / 1991 / Protein engineering of singlechain Fv analogs and fusion proteins / Methods Enzymol 203 : 46 ~ 88
5. [단행본] - Kabat EA / 1991 / Sequences of proteins of immunological interest / NIH publication : 91 ~ 3242
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UCI(KEPA) : I410-ECN-0101-2013-527-001397698