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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.4
- 수록면
- 357 - 361 (5page)
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초록· 키워드
오류제보하기
One of the major allergenic proteins in common buckwheat (Fagopyrum elculentum) was found to be a BW10KD. In this work, allergenic BW10KD genomic DNAs from the native common buckwheat ‘Pyeongchang’ and Tartarian buckwheat ‘Clfa47’ were cloned by polymerase chain reaction (PCR), and their nucleotide sequences were determined. In addition, a novel PCR assay targeting the allergenic BW10KD gene was developed to detect and differentiate both buckwheat species in food. The nucleotide sequences of the BW10KD genomic DNA from ‘Pyeongchang’ and ‘Clfa47’ were 94% identical. Base differences in the nucleotide sequences of the BW10KD genes are probably useful as a molecular marker for species-specific identification. The ‘Pyeongchang’-specific primer set 154PF/400PR and the ‘Clfa47’-specific primer set 154DF/253DR generated 247 and 100 bp fragments in singleplex PCR, respectively. A duplex PCR assay with 2 species-specific primer sets simultaneously differentiated the ‘Pyeongchang’ and ‘Clfa47’ in a single reaction. The PCR assay also successfully allowed for the rapid detection of buckwheat ingredients in foods.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgment
References
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Li SQ / 2001 / Advances in the development of functional foods from buckwheat / Crit. Rev. Food Sci 41 : 451 ~ 464
2. [학술지(정기간행물)] - Yoshimasu MA / 2000 / Electrophoretic and immunochemical characterization of allergenic proteins in buckwheat / Int. Arch. Allergy Imm 123 : 130 ~ 136
3. [학술지(정기간행물)] - Fujino K / 2001 / Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins / J. Agr. Food Chem 49 : 1825 ~ 1829
4. [학술지(정기간행물)] - Park JW / 2000 / Identification and characterization of the major allergens of buckwheat / Allergy 55 : 1035 ~ 1041
5. [학술지(정기간행물)] - Wieslander G / 001 / Buckwheat allergy / Allergy 56 : 703 ~ 704
2. [학술지(정기간행물)] - Yoshimasu MA / 2000 / Electrophoretic and immunochemical characterization of allergenic proteins in buckwheat / Int. Arch. Allergy Imm 123 : 130 ~ 136
3. [학술지(정기간행물)] - Fujino K / 2001 / Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins / J. Agr. Food Chem 49 : 1825 ~ 1829
4. [학술지(정기간행물)] - Park JW / 2000 / Identification and characterization of the major allergens of buckwheat / Allergy 55 : 1035 ~ 1041
5. [학술지(정기간행물)] - Wieslander G / 001 / Buckwheat allergy / Allergy 56 : 703 ~ 704
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UCI(KEPA) : I410-ECN-0101-2013-573-001015486