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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.4
- 수록면
- 279 - 286 (8page)
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초록· 키워드
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The antioxidative activity of various enzymatic extracts from leaves of Perilla frutescens var. japonica was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the leaves were enzymatically hydrolyzed by 8 carbohydrases (Dextrozyme, AMG, Promozyme, Maltogenase, Termamyl, Viscozyme, Celluclast, and BAN) and 9 proteases [Flavourzyme, Neutrase, Protamex, Alcalase, PP-trypsin (trypsin from porcine pancreas), papain, pepsin, α-chymotrypsin, and BP-trypsin (trypsin from bovine pancreas)]. The DPPH radical scavenging activities of Promozyme and Alcalase extracts were the highest, and the IC<sub>50</sub> values were 77.25 and 109.66 μg/mL, respectively. All enzymatic extracts of the leaves scavenged hydroxyl radical, and the IC<sub>50</sub> values of Celluclast and pepsin extracts which were the highest activity were 243.34 and 241.86 μg/mL, respectively. The BAN and α-chymotrypsin extracts showed the highest scavenging activities, and the IC<sub>50</sub> values were 21.13 and 33.23 μg/mL, respectively. The pepsin extracts from the leaves showed protective effect on H<sub>2</sub>O<sub>2</sub>-induced DNA damage. In addition, the pepsin extracts decreased cell death in PC-12 cells against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of the leaves possess antioxidative activity.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
참고문헌 (43)
참고문헌 신청
1. [학술지(정기간행물)] - Sheu SS / 2006 / Targeting antioxidants to mitochondria: A new therapeutic direction / Biochim. Biophys. Acta 1762 : 256 ~ 265
2. [단행본] - Cashman JR / 1997 / Monoamine oxidase and flavin-containing monooxygenases. In: Biotransformation / Elsevier : 69 ~ 96
3. [학술지(정기간행물)] - Muramatsu H / 1995 / Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility / Cancer Res 55 : 6210 ~ 6214
4. [학술지(정기간행물)] - Leanderson P / 1997 / Green tea polyphenols inhibit oxidant-induced DNA strand breakage in cultured lung cells / Free Radical Bio. Med 23 : 235 ~ 242
5. [학술지(정기간행물)] - Sussman MS / 1990 / Oxygen-derived free radicals in reperfusion injury / Method Enzymol 186 : 711 ~ 723
2. [단행본] - Cashman JR / 1997 / Monoamine oxidase and flavin-containing monooxygenases. In: Biotransformation / Elsevier : 69 ~ 96
3. [학술지(정기간행물)] - Muramatsu H / 1995 / Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility / Cancer Res 55 : 6210 ~ 6214
4. [학술지(정기간행물)] - Leanderson P / 1997 / Green tea polyphenols inhibit oxidant-induced DNA strand breakage in cultured lung cells / Free Radical Bio. Med 23 : 235 ~ 242
5. [학술지(정기간행물)] - Sussman MS / 1990 / Oxygen-derived free radicals in reperfusion injury / Method Enzymol 186 : 711 ~ 723
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UCI(KEPA) : I410-ECN-0101-2013-573-001015360