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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.4
- 수록면
- 519 - 525 (7page)
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초록· 키워드
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A bacterial strain (Strain N-08) capable of extracellularly producing high level of non-reducing oligosaccharide (NR-OS) isolated from soil. The strain was identified phylogenetically by 16S rDNA sequence analysis and found to be very close to Arthrobacter crystallopoietes. The high production of NR-OS was observed in the basal culture medium containing maltose as a sole carbon source. The NR-OS in culture supernatant was purified by glucoamylase treatment and Dowex-1 (OH?) ion exchange chromatography and its structure was characterized. This oligosaccharide consisted of only glucose. Methylation analysis indicated that this fraction was composed mainly of non-reducing terminal glucopyranoside. Matrixassisted laser-induced/ionization time-of-flight (MALDI-TOF) and electrospray ionization-mass spectrometry (ESI-MS)/MS analyses suggested that this oligosaccharide comprised non-reducing disaccharide unit with 1,1-glucosidic linkage. When this disaccharide was analyzed by ¹H-NMR and ¹³C-NMR, it gave the same signals with α-D-glucopyranosyl-(1,1)-α-Dglucopyranoside. These results indicated that the NR-OS produced by A. crystallopoietes N-08 was α1,α1-trehalose. This is the first report of the trehalose which can be produced directly from maltose by A. crystallopoietes N-08.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
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