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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.16 No.4
- 발행연도
- 2011.12
- 수록면
- 357 - 363 (7page)
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Recently, we found that Arthrobacter crystallopoietes N-08 isolated from soil directly produces trehalose from maltose by a resting cell reaction. In this study, the optimal set of conditions and substrate specificity for the trehalose production using resting cells was investigated. Optimum temperature and pH of the resting cell reaction were 55℃ and pH 5.5, respectively, and the reaction was stable for two hours at 37～55℃ and for one hour at the wide pH ranges of 3～9. Various disaccharide substrates with different glycosidic linkages, such as maltose, isomaltose, cellobiose, nigerose, sophorose, and laminaribiose, were converted into trehalose-like spots in thin layer chromatography (TLC). These results indicated broad substrate specificity of this reaction and the possibility that cellobiose could be converted into other trehalose anomers such as α,β- and -trehalose. Therefore, the product after the resting cell reaction with cellobiose was purified by -glucosidase treatment and Dowex-1 (OH?) column chromatography and its structure was analyzed. Component sugar and methylation analyses indicated that this cellobiose-conversion product was composed of only non-reducing terminal glucopyranoside. MALDI-TOF and ESI-MS/MS analyses suggested that this oligosaccharide contained a non-reducing disaccharide unit with a 1,1-glucosidic linkage. When this disaccharide was analyzed by ¹H-NMR and <SUP>13</SUP>C-NMR, it gave the same signals with α-D-glucopyranosyl-(1,1)-α-D-glucopyranoside. These results suggest that cellobiose can be converted to α,α-trehalose by the resting cells of A. crystallopoietes N-08.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (33)
참고문헌 신청
1. [학술지(정기간행물)] - Wingler A / 2002 / The function of trehalose biosynthesis in plants / Phytochemistry 60 : 437 ~ 440
2. [학술지(정기간행물)] - Elbein AD / 2003 / New insights on trehalose: a multifunctional molecule / Glycobiology 13 : 17 ~ 27
3. [학술지(정기간행물)] - Thevelein JM / 1984 / Regulation of trehalose mobilization in fungi / Microbiol Rev 48 : 42 ~ 59
4. [학술지(정기간행물)] - Lillie SH / 1980 / Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation / J Bacteriol 143 : 1384 ~ 1394
5. [학술지(정기간행물)] - Kandror O / 2002 / Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures / Proc Natl Acad Sci USA 99 : 9727 ~ 9732
2. [학술지(정기간행물)] - Elbein AD / 2003 / New insights on trehalose: a multifunctional molecule / Glycobiology 13 : 17 ~ 27
3. [학술지(정기간행물)] - Thevelein JM / 1984 / Regulation of trehalose mobilization in fungi / Microbiol Rev 48 : 42 ~ 59
4. [학술지(정기간행물)] - Lillie SH / 1980 / Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation / J Bacteriol 143 : 1384 ~ 1394
5. [학술지(정기간행물)] - Kandror O / 2002 / Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures / Proc Natl Acad Sci USA 99 : 9727 ~ 9732
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UCI(KEPA) : I410-ECN-0101-2013-511-001347376