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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2008.11
- 수록면
- 68 - 71 (4page)
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We investigated the effect of herbal extract of Dryopteris crassirhizoma on the cell viability, immune response, and nitric oxide production of chicken splenocyte. Nitrite levels were assayed in the culture supernatant of splenocyte after 24h by the Griess method. The expressions of pro-inflammatory cytokines such as interleukin (IL-2, IL-6, IL-8, IL-18) and the housekeeping gene GAPDH were studied using RT-PCR. Splenocyte were cultured with the herbal extracts at 37℃ in 96-well plate for 24h. Not cytotoxic effect was observed at 20 ㎍ and 40 ㎍. The NO production increased dose dependently with the treatment of herbal extract. which suggest that this herbal extract has no anti-inflammatory effect. In cytokine expression it was observed that the herbal extract induced the expression of IL-2 and suppressed the expression of IL-6 and IL-18, however there was no effect on the expression of IL-8. It may be concluded that the Dryopteris crassirhizoma extract is non toxic and have no anti-inflammatory effect and it has immune stimulation effect through IL-2.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULT AND DISCUSSION
Reference
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