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To clerify the antioxidant effect of Crataegi Fructus (CF) extract on reactive oxygen species (ROS), The C6 glioma cells were treated with various concentrations of hydrogen peroxide (H₂O₂). The H₂O₂-induced neurotoxicity was measured by XTT assay for the cell viability. For the protective effect of CF extract on the cytotoxicity induced by H₂O₂, cell viability, lactate dehydroganase (LDH) activity, and the inhibitive activity of lipid peroxidation of CF extract were performed. In this study, H₂O₂ decreased cell viability dose- and time-dependent manners and increased LDH activity compared with the control. In the protective effect on H₂O₂, CF extract increased cell viability and decreased LDH activity on H₂O₂-induced cytotoxicity, lipid peroxidation by FTC assay. From these results, It is suggested that H₂O₂ was highly toxic on cultured C6 glioma cells, and also, CF extract showed the protective effect on H₂O₂-mediated cytotoxicity.
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UCI(KEPA) : I410-ECN-0101-2010-510-001462628