지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
열충격단백질70 (HSP70)은 복수유전자족으로서 통상적으로 표현되는 Hsc70와 스트레스에 의하여 유도되는 Hsp70가 있다. 포유동물의 신경계통에서는 상당한 량의 HSP70가 정상조건에서도 표현되는 것으로 알려져 있다. 본 연구에서는 흰쥐의 소뇌 세포의 연접에서 Hsp70의 표현에 대한 연구를 하였다. 면역조직화학적으로 소뇌절편을 염색하여 관찰한 결과 Hsp70와 Hsc70 모두 표현되었는데, 소뇌 조롱박세포에서 가장 강하게 표현되었으며, 다음으로 소뇌 과립세포에서 강하게 표현되었다. 또한 깊은소뇌핵의 신경세포들도 강하게 염색되었다. 배양한 P1 소뇌신경세포를 Hsp70 항체로 염색한 결과 Hsp70는 조롱박세포와 과립세포에서 모두 표현되었으며, 세포체와 가지돌기를 따라 점박이를 형성하였다. 이들 점박이들은 PSD95 점박이와 같이 위치하였다. 그리고 PSD 분획을 이용한 면역염색에서도 PSD70이 검출되었다. 본 연구결과는 Hsp70이 정상조건에서도 소뇌신경세포의 연접에 존재함을 의미한다.
목차
Abstract
Materials and Methods
Results and Discussion
Acknowledgment
References
초록
참고문헌 (20)
참고문헌 신청
Brewer, G. J., J. R. Torricelli, E. K. Evege and P. J. Price. 1993. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci. Res. 35, 567-576.
Brown, I. R 1990. Induction of heat shock (stress) genes in the mammalian brain by hyperthermia and other traumatic events: a current perspective. J. Neurosci. Res. 27, 247-255.
Engert, F., T. Bonhoeffer. 1999. Dendritic spine changes associated with hippocampal long-term synaptic plasticity. Nature 399, 66-70.
Feige U, B. S. Polla. (1994) Heat shock proteins: the HSP70 family. Experientia 50, 979-986.
Hosokawa, T., D. A. Rusakov, T. V. P. Bliss and A. Fine. 1995. Repeated confocal imaging of individual dendritic spines in the living hippocampal slice: evidence for changes in length and orientation associated with chemically induced LTP. J. Neurosci. 15
Brown, I. R 1990. Induction of heat shock (stress) genes in the mammalian brain by hyperthermia and other traumatic events: a current perspective. J. Neurosci. Res. 27, 247-255.
Engert, F., T. Bonhoeffer. 1999. Dendritic spine changes associated with hippocampal long-term synaptic plasticity. Nature 399, 66-70.
Feige U, B. S. Polla. (1994) Heat shock proteins: the HSP70 family. Experientia 50, 979-986.
Hosokawa, T., D. A. Rusakov, T. V. P. Bliss and A. Fine. 1995. Repeated confocal imaging of individual dendritic spines in the living hippocampal slice: evidence for changes in length and orientation associated with chemically induced LTP. J. Neurosci. 15
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UCI(KEPA) : I410-ECN-0101-2009-470-015375401