지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 403 - 409 (7page)
이용수
초록· 키워드
오류제보하기
Saccharomyces cerevisiae Hsp30 is a plasma membrane heat shock protein that is induced by various environmental stress conditions. However, the functional role of Hsp30during diverse environmental stressors is not presently known. To gain insight into its function during thermal stress, we have constructed and characterized a Δhsp30strain during heat stress. BY4741Δhsp30 cells were found to be more sensitive compared with BY4741 cells, when exposed to a lethal heat stress at 50oC. When budding yeast is exposed to either heat shock or weak organic acid,it inhibits Pma1p activity. In this study, we measured the levels of Pma1p in mutant and Wt cells both during optimal temperature and heat shock temperature. We observed that BY4741Δhsp30 cells showed constitutive reduction of Pma1p. To gain further insights into the role of Hsp30 during heat stress, we compared the total protein profile by 2D gel electrophoresis followed by identification of differentially expressed spots by LC-MS. We observed that contrary to that expected from thermal-stress-induced changes in gene expression, the Δhsp30 mutant maintained elevated levels of Pdc1p, Trx1p, and Nbp35p and reduced levels of Atp2p and Sod1p during heat shock. In conclusion,Hsp30 is necessary during lethal heat stress, for the maintenance of Pma1p and a set of thermal stress response functions.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Adams, A / 1997 / Methods in Yeast Genetics / Cold Spring Harbour Laboratory Press
Baudin, A / 1993 / A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae / Nucleic Acid Res 21 : 3329 ~ 3330
Braley, R / 1997 / The C-terminus of yeast plasma membrane H+-ATPase is essential for the regulation of this enzyme by heat shock protein Hsp30, but not for stress activation
Galeote, V. A / 2007 / Sfl1p as an activator of HSP30 gene in Saccharomyces cerevisiae / Curr. Genet 52 : 55 ~ 63
Gasch, A. P / 2000 / Genomic expression programs in the response of yeast cells to environmental change / Mol. Biol. Cell 11 : 4241 ~ 4257
Baudin, A / 1993 / A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae / Nucleic Acid Res 21 : 3329 ~ 3330
Braley, R / 1997 / The C-terminus of yeast plasma membrane H+-ATPase is essential for the regulation of this enzyme by heat shock protein Hsp30, but not for stress activation
Galeote, V. A / 2007 / Sfl1p as an activator of HSP30 gene in Saccharomyces cerevisiae / Curr. Genet 52 : 55 ~ 63
Gasch, A. P / 2000 / Genomic expression programs in the response of yeast cells to environmental change / Mol. Biol. Cell 11 : 4241 ~ 4257
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Two-signal blockade with anti-CD45RB and anti-CD154 monoclonal antibodies inhibits graft rejection via CD4-dependent mechanisms in allogeneic skin transplantation
Experimental and Molecular Medicine
2006 .01
-
The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress
Experimental and Molecular Medicine
2006 .01
-
Analysis of the Correlation between Expressions of HSP90α, HSP90β, and GRP94, and the Clinicopathologic Characteristics in Tissues of Non-Small Cell Lung Cancer Patients
대한임상검사과학회지
2017 .01
-
Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells
Experimental and Molecular Medicine
2006 .01
-
Heat Shock Protein 90 (HSP90) and Immune Regulation
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015 .03
-
Differential expression of two stress-inducible hsp70 genes by various stressors
Experimental and Molecular Medicine
2002 .01
-
Inhibition of Citrate Synthase Thermal Aggregation In Vitro by Recombinant Small Heat Shock Proteins
Journal of Microbiology and Biotechnology
2009 .01
-
The Inducible form of Heat Shock Protein 70 (Hsp70) is Expressed in the Rat Cerebellar Synapses in Normal Condition
생명과학회지
2005 .08
-
Unleashing the full potential of Hsp90 inhibitors as cancer therapeutics through simultaneous inactivation of Hsp90, Grp94, and TRAP1
Experimental and Molecular Medicine
2020 .01
-
넙치(Paralichthys olivaceus) HSP90β 유전자의 분자생물학적 연구
한국미생물·생명공학회지
2004 .01
-
Bioinformatics Analysis of Hsp20 Sequences in Proteobacteria
Genomics & informatics
2009 .01
-
Apostichopus japonicas (Echinodermata; Holothuroidea)에서 온도 스트레스에 의한 Hsp90 및 Ferritin 유전자의 발현
환경생물
2015 .01
-
Changes of Gene Expression in NIH3T3 Cells Exposed to Osmotic and Oxidative Stresses
Genomics & informatics
2004 .01
-
SIRT1 Inhibitor Enhances Hsp90 Inhibitor-mediated Abrogation of Hsp90 Chaperone Function and Potentiates the Cytotoxicity of Hsp90 Inhibitor in Chemo-resistant Human Cancer Cells
생명과학회지
2016 .07
-
Novel Modification of Growth Medium Enables Efficient E. coli Expression and Simple Purification of an Endotoxin-Free Recombinant Murine Hsp70 Protein
Journal of Microbiology and Biotechnology
2009 .01
-
ERK-dependent phosphorylation of the linker and substrate-binding domain of HSP70 increases folding activity and cell proliferation
Experimental and Molecular Medicine
2019 .01
-
Effects of a mild heat treatment on mouse testicular gene expression and sperm quality
Animal cells and systems
2010 .01
-
CD36 signaling inhibits the translation of heat shock protein 70 induced by oxidized low density lipoprotein through activation of peroxisome proliferators-activated receptor γ
Experimental and Molecular Medicine
2008 .01
-
Vascular Smooth Muscle Cells Secrete CXCL10 in Response to Heat Shock Protein 90
생명과학회지
2011 .05
-
Protein kinase Cμ plays an essential role in hypertonicity-induced heat shock protein 70 expression
Experimental and Molecular Medicine
2008 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0