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Metabolic Engineering of Saccharomyces cerevisiae to Improve Glucan Biosynthesis
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논문 기본 정보

Type
Academic journal
Author
Journal
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제5호 KCI Accredited Journals SCOPUS
Published
2019.1
Pages
758 - 764 (7page)

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Metabolic Engineering of Saccharomyces cerevisiae to Improve Glucan Biosynthesis
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Abstract· Keywords

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β-Glucan is a chief structural polymer in the cell wall of yeast. β-Glucan has attracted intensive attention because of its wide applications in health protection and cosmetic areas. In the present study, the β-glucan biosynthesis pathway in S. Cerevisiae was engineered to enhance β-glucan accumulation. A newly identified bacterial β-1, 6-glucan synthase GsmA from Mycoplasma agalactiae was expressed, and increased β-glucan content by 43%. In addition, other pathway enzymes were investigated to direct more metabolic flux towards the building of β-glucan chains. We found that overexpression of Pgm2 (phosphoglucomutase) and Rho1 (a GTPase for activating glucan synthesis) significantly increased β-glucan accumulation. After further optimization of culture conditions, the β-glucan content was increased by 53.1%. This study provides a new approach to enhance β-glucan biosynthesis in Saccharomyces cerevisiae.

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References (29)

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Barsanti L / 2011 / Chemistry, physico-chemistry and applications linked to biological activities of beta-glucans / Nat. Prod. Rep 28:457~466 google schola Samuelsen ABC / 2014 / Effects of orally administered yeast-derived beta-glucans : a review / Mol. Nutr. Food Res 58:183~193 google schola Kayali H / 2005 / The antioxidant effect of β-glucan on oxidative stress status in experimental spinal cord injury in rats / Neurosurg. Rev 28:298~302 google schola Du B / 2014 / Skin health promotion effects of natural beta-glucan derived from cereals and microorganisms : a review / Phytother. Res 28:159~166 google schola Dalonso N / 2015 / β-(1→3), (1→6)-Glucans : medicinal activities, characterization, biosynthesis and new horizons / Appl. Microbiol. Biotechnol 99:7893~7906 google schola

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