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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
To investigate the antioxidant activity of extract from the raw walnut, Juglans sinensis Dode, we prepared five fractions (methanol (MeOH), dichloromethane (CH₂Cl₂), ethyl acetate (EtOAc), n-buthanol (n-BuOH) and dehydrogen monooxide (H₂O) fractions) and examined. The effect of walnut extract on the oxidative stress was investigated in vitro. The DPPH (2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl) free radical scavenging activity of extract from raw walnut was shown in the following order: EtOAc fraction＞n-BuOH fraction＞MeOH fraction＞CH₂Cl₂fraction＞H₂O layer. The result showed that the highest activity (0.56 ㎍/ml, IC_(50).) was observed in EtOAc fraction, whereas n-BuOH fraction, MeOH fraction, CH₂Cl₂fraction and H₂O layer of IC_(50) were 2.34 ㎍/ml, 3.88 ㎍/ml, 8.06 ㎍/ml, and 8.19 ㎍/ml, respectively. The radical scavenging activity assay of each fraction showed that the antioxidative activity was observed in the following order: EtOAc fraction (74.27±1.56%)＞MeOH fraction (60.76±3.4%)＞n-BuOH fraction (59.32±0.88%)＞H₂O layer (41.69±2.06%). These results revealed that all fractions, except for CH₂Cl₂fraction, showed high antioxidative activity. Furthermore, the peroxynitrite (ONOO-) scavenging activity was assayed in each fraction. The result showed that the ONOO- scavenging activity of EtOAc fraction, MeOH fraction and n-BuOH fraction from raw walnut was 95.14±0.36%, 90.02±1.19% and 89.41±0.81%, respectively. The tert-butylhydroperoxide (t-BHP) treatment in vitro increased lactate dehydrogenase release and lipid peroxidation in renal cortical slices. Such changes were completely prevented by addition of MeOH fraction, EtOAc fraction and n-BuOH fraction of walnut. These results indicate that the walnut extract exerts the benedicial effect against t-BHP-induced cell injury and its effect may be due to antioxidant action. In addition, it is suggested that walnut extract might be developed as the effective scavenger for the prevention of oxidative stress.
목차
Abstract
재료 및 방법
결과 및 고찰
요약
감사의 글
참고문헌
참고문헌 (24)
참고문헌 신청
Balavoine, G. G. and Y. V. Genleti. 1999. Peroxynitrite scavenging by different antioxidants, PartⅠ: convenient assay. Nitric Oxide. 3, 40-54.
Bondet, V., W. Brand Williams and C. Berset. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radecal method, Lebensm.-Wiss. U-Technol. 30, 609-615.
Branen, A. S. 1975. Toxicology and biochemistry of butylated hydroxyanisole and buthylated hydroxytoluene. JAOCS. 1, 59-62
Ahn, C. B., C. H. Song, W. H. Kim and Y. K. Kim. 2002. Effects of Juglans sinensis Dode extract and antioxidant on mercury chloride induced acute renal failure in rabbits. Journal of Ethnopharmacology. 82, 45-49
Choi, H. R., J. S. Choi, Y. N. Han, S. J. Bae and H. Y. Chung. 2002. Peroxynitrite scavenging activity of herb extracts. Phytother. Res. 16, 232-235,
Bondet, V., W. Brand Williams and C. Berset. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radecal method, Lebensm.-Wiss. U-Technol. 30, 609-615.
Branen, A. S. 1975. Toxicology and biochemistry of butylated hydroxyanisole and buthylated hydroxytoluene. JAOCS. 1, 59-62
Ahn, C. B., C. H. Song, W. H. Kim and Y. K. Kim. 2002. Effects of Juglans sinensis Dode extract and antioxidant on mercury chloride induced acute renal failure in rabbits. Journal of Ethnopharmacology. 82, 45-49
Choi, H. R., J. S. Choi, Y. N. Han, S. J. Bae and H. Y. Chung. 2002. Peroxynitrite scavenging activity of herb extracts. Phytother. Res. 16, 232-235,
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