지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Infection with hepatitis B virus (HBV) is a major health problem worldwide. Although a tremendous amount has been known about HBV, there have been obstacles in the study of HBV due to the narrow host range of HBV limited to humans and primates. In the present study, we investigated the susceptibility to HBV infection of mouse hepatoma cell line, Hepa-1c1c7. In addition, based on that human hepatocytes infected by HBV increase the expression of the pro-inflammatory cytokine TNF-a, the inducibility of TNF-a expression by HBV in the cells was determined. HBV surface antigen (HBsAg) secretion was measured by the microparticle enzyme immunoassay and steady state mRNA expression was analyzed by quantitative competitive RT-PCR. Transient transfection of Hepa-1c1c7 cells with HBV expression vector resulted in a dose-dependent induction of TNF-a expression. Infection of Hepa-1c1c7 cells with the serum of HBV carrier also increased TNF-a mRNA expression. Both in the transfected and infected cells, HBV mRNA was expressed and significant HBsAg secretion was detected. There was no significant variation in β-actin mRNA expression by HBV. These results demonstrate that HBV is infectious to Hepa-1c1c7 in vitro and the viral infection induces TNF-a expression, which suggests that Hepa-1c1c7, a mouse hepatoma cell line, may be a possible model system for analysis of various molecular aspects of HBV infection.
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Abstract
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참고문헌
참고문헌 (31)
참고문헌 신청
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Bchini R, F. Capel, C. Dauguet, S. Dubanchet and M. Petit. 1990. In vitro infection of human hepatoma (Hep-G2) cells with hepatitis B virus. J. Virol. 64, 3025-3032.
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Bradham C. A., J. Plumpe, M. P. Manns, D. A. Brenner and C. Trautwein. 1998. Mechanisms of hepatic toxicity. I. TNF-induced liver injury. Am. J. Physiol. 275, G387-392.
Chisari F. V. 1996. Hepatitis B virus transgenic mice: models of viral immunobiology and pathogenesis. Curr. Top. Microbiol. Immuol. 206, 149-173.
Bchini R, F. Capel, C. Dauguet, S. Dubanchet and M. Petit. 1990. In vitro infection of human hepatoma (Hep-G2) cells with hepatitis B virus. J. Virol. 64, 3025-3032.
Beasley R. P., L. Y. Hwang, C. C. Lin and C. S. Chien. 1981. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22,707 men in Taiwan. Lancet 2, 1129-1133.
Bradham C. A., J. Plumpe, M. P. Manns, D. A. Brenner and C. Trautwein. 1998. Mechanisms of hepatic toxicity. I. TNF-induced liver injury. Am. J. Physiol. 275, G387-392.
Chisari F. V. 1996. Hepatitis B virus transgenic mice: models of viral immunobiology and pathogenesis. Curr. Top. Microbiol. Immuol. 206, 149-173.
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UCI(KEPA) : I410-ECN-0101-2009-470-015374484