지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
This study was conducted to compare the immunochemical properties of female-specific serum proteins (vitellogenin, VTG) and egg yolk proteins in female fusilier, Caesio diagramma.
VTG of fusilier was identified and characterized by using immunochemical analysis. Two types of VTG (VTG1 and VTG2) reacted clearly with antiserum against egg proteins, were confirmed in the serum of mature female. The results of sephacryl S-300 showed that the molecular weights of VTG1 and VTG2 were 560,000 and 410,000, respectively.
Yolk proteins, E2 and E3, were isolated from egg extracts, and molecular weights of them were estimated 410,000 and 170,000, respectively.
The treatment of 17β-estradiol (E₂) to males has induced the synthesis of VTG of which immunological characteristics seems to be similar to the yolk proteins. The results suggest that VTG can be synthesized in the liver by the action of E₂ stimulation, and incorporated into the oocytes through the blood circulation. The level of serum E₂ was moderately high throughout the spawning period of June. The level of serum VTG was also sustained at high in May and June. The concentration changes of serum E₂ and VTG were corelated to the ovarian development in female fusilier.
The results indicated that E₂ may have some important roles for the vitellogenesis in female fusilier. Also, the VTG can be a precursor protein of yolk not only because it could be synthesized in the liver then incorporated into the oocytes but also because an egg yolk protein had the similiar molecular weights and antigenecity with VTG.
VTG of fusilier was identified and characterized by using immunochemical analysis. Two types of VTG (VTG1 and VTG2) reacted clearly with antiserum against egg proteins, were confirmed in the serum of mature female. The results of sephacryl S-300 showed that the molecular weights of VTG1 and VTG2 were 560,000 and 410,000, respectively.
Yolk proteins, E2 and E3, were isolated from egg extracts, and molecular weights of them were estimated 410,000 and 170,000, respectively.
The treatment of 17β-estradiol (E₂) to males has induced the synthesis of VTG of which immunological characteristics seems to be similar to the yolk proteins. The results suggest that VTG can be synthesized in the liver by the action of E₂ stimulation, and incorporated into the oocytes through the blood circulation. The level of serum E₂ was moderately high throughout the spawning period of June. The level of serum VTG was also sustained at high in May and June. The concentration changes of serum E₂ and VTG were corelated to the ovarian development in female fusilier.
The results indicated that E₂ may have some important roles for the vitellogenesis in female fusilier. Also, the VTG can be a precursor protein of yolk not only because it could be synthesized in the liver then incorporated into the oocytes but also because an egg yolk protein had the similiar molecular weights and antigenecity with VTG.
목차
Abstract
Introduction
Materials and methods
Results
Discussion
요약
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Immunoblotting Reactivity of Vitellogenin Antibodies against Native Vitellogenins and a Vitellogenin Protein Fragment Produced in E. coli
Journal of applied biological chemistry
2009 .01
-
무지개송어(Oncorhynchus mykiss) 배양 간세포에서 Vitellogenin 합성에 미치는 Cu 및 Zn의 억제효과
한국양식학회지
1998 .08
-
무지개송어(Oncorhynchus mykiss) 배양 간세포에서 Vitellogenin 합성에 미치는 Calcium ionophore의 영향
한국양식학회지
1998 .05
-
Reproductive Cycle in Female Fusilier, Caesio diagramma
한국양식학회지
1996 .02
-
Identification, Purification, and Immunoassay of Stone Flounder (Kareius bicolouratus) Vitellogenin
Applied Biological Chemistry
2012 .01
-
Aluminum Inhibits Vitellogenin Production via Toxic Effects on Hepatocytes in the Rockfish Sebastes schlegelii
Fisheries and Aquatic Sciences
2011 .01
-
황점볼락 난소 발달에 따른 혈중 성호르몬과 난황단백전구체의 변동
한국양식학회지
2009 .02
-
Effects of High Intracellular Calcium Concentration by Ouabain on VTG Production in the Primary Hepatocyte Cultures of Rainbow Trout, Oncorhynchus mykiss.
한국양식학회지
1998 .05
-
Rockfish (Sebastes schlegeli) Vitellogenin: Purification, Characterization and Development of Sandwich ELISA System
Fisheries and Aquatic Sciences
2004 .01
-
참전복(Haliotis discus hannai)의 난황단백질 분리와 특성
한국양식학회지
1998 .05
-
무지개송어, Oncorhynchus mykiss 배양 간세포에서 2, 4-Dichlorophenoxy acetic acid이 Vitellogenin 합성과 E₂-ER Binding affinity에 미치는 영향
한국양식학회지
2002 .02
-
Antioxidative and Anti-Inflammatory Activity of Egg Yolk Protein
한국식품영양과학회 학술대회발표집
2018 .10
-
Quantitative Comparison of Radiation-induced Hydrocarbons from Irradiated Raw Egg Yolk, Boild Egg Yolk and Egg Yolk Powder
Food Science and Biotechnology
2003 .12
-
Sequential Separation of Main Components from Chicken Egg Yolk
Food Science and Biotechnology
2006 .04
-
무지개송어의 간세포 초대배양에 의한 Vitellogeinin 합성 유도
한국양식학회지
1998 .11
-
참돔(Pagrus major)의 혈장 卵黃蛋白前驅體에 대한 酵素免疫測定法과 卵巢成熟의 早期判定
한국양식학회지
1995 .02
-
넙치(Paralichthys olivaceus) 배발생동안 인슐린유사성장인자와 비텔로제닌의 발현
수산해양교육연구
2019 .04
-
Egg Yolk Reduce Blood Lipid in Normal Rats
한국식품영양과학회 학술대회발표집
2015 .08
-
The Protective Effect of Egg Yolk Protein on Stress-Induced Immunodeficiency in Balb/c Mouse
한국식품영양과학회 학술대회발표집
2019 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-529-015320444