지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 219 - 227 (9page)
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초록· 키워드
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Vitellogenin (Vtg), the precursor of egg yolk proteins, serves as nutrients for the developing embryos. Vtg production in male fish has become an excellent biomarker for environmental estrogens. Thus, Vtg synthesis was induced by injection of $17{\beta}$-estradiol ($E_2$) in stone flounder (Kareius bicolouratus). Plasma from uninduced and $E_2$-treated fish was subjected to native polyacrylamide gel electrophoresis (PAGE) and the gels were stained with Coomassie brilliant blue R250 for proteins, methyl green for phosphates, Sudan black B for lipids, and periodic acid-Schiff's reagent for carbohydrates. These analyses verified the presence of two phospholipoglycoproteins in the plasma from $E_2$-treated stone flounder, which were tentatively identified as Vtg. Vtg was then isolated by two purification procedures: gel filtration followed by anion exchange chromatography, or selective precipitation combined with anion exchange chromatography. The latter procedure was more effective at isolating Vtg with a high degree of purity. In native PAGE, the apparent molecular masses of Vtgs were determined to be 520 and 550 kDa, respectively. Two major bands with approximate molecular masses of 165 and 106 kDa together with two additional faint bands of lower molecular weights were observed after sodium dodecyl sulfate-PAGE under reducing conditions. These bands exhibited differential affinity for polyclonal antisera against red drum (Sciaenops ocellatus) Vtg in Western blot. The present study established the purification procedure and characterized the electrophoretic and immunological properties of stone flounder Vtg.
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