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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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In red seabream, Pagrus major the female specific protein in the vitellogenic female serum was identified by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The female specific serum protein might be vitellogenin based on the results of the immunological analysis for the male and vitellogenic female sera and crude egg extracts. Also, it was identified by the immunodiffusion test that the purified yolk protein from ovarian egg extracts has antigenic identities shared with the female specific serum protein.
To study the relationship between the maturational stages of gonad and plasma levels of vitellogenin, these were measured from the late resting period (January) to the vitellogenic preiod (April) by the modified enzymeimmunoassay (EIA) using antisenun against yolk protein. The level of plasma vitellogenin began to increase in February (previtellogenesis stage) and continuously increased with the ovarian growth during the vitellogenesis period (March to April). The plasma vitellogenin levels were significantly different between the females and the males in February.
Validation for the modified EIA system was tested. The absorbance curve of serial dilutions of serum from the vitellogenic female was paralleled to the standard curve of yolk protein ; 109±5.6% recovery was achieved by the modified ETA. And the intraassay coefficients of variation were less than 10% within the concentration ranging from 31.3 ng / ㎖ to 1,000 ng /㎖.
These findings suggest that the sex determination in adult red seabreams could be possible by using the modified EIA as early as in February.
To study the relationship between the maturational stages of gonad and plasma levels of vitellogenin, these were measured from the late resting period (January) to the vitellogenic preiod (April) by the modified enzymeimmunoassay (EIA) using antisenun against yolk protein. The level of plasma vitellogenin began to increase in February (previtellogenesis stage) and continuously increased with the ovarian growth during the vitellogenesis period (March to April). The plasma vitellogenin levels were significantly different between the females and the males in February.
Validation for the modified EIA system was tested. The absorbance curve of serial dilutions of serum from the vitellogenic female was paralleled to the standard curve of yolk protein ; 109±5.6% recovery was achieved by the modified ETA. And the intraassay coefficients of variation were less than 10% within the concentration ranging from 31.3 ng / ㎖ to 1,000 ng /㎖.
These findings suggest that the sex determination in adult red seabreams could be possible by using the modified EIA as early as in February.
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UCI(KEPA) : I410-ECN-0101-2009-529-015332108