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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.4
- 수록면
- 418 - 425 (8page)
- DOI
- 10.13000/JFMSE.2019.4.31.2.418
이용수
초록· 키워드
오류제보하기
This study investigated the expression of insulin-like growth factor(IGF) and vitellogenin(Vtg) during embryonic development in the olive flounder(Paralichthys olivaceus). Morphological stages were divided into four developments as prehatching, hatching, 1 day after hatching, and 2 days after hatching. During embryonic development, expression of mRNA and protein of IGF-I, IGF-II, IGF-I receptor(IGF-IR), and Vtg were investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western-blot analysis. During development progresses, the IGF-II mRNA level was higher expressed than that of IGF-I. In addition, the level of IGF-II and IGF-IR mRNA had highest value in the hatching period. The Vtg mRNA showed a very similar pattern. Meanwhile, the IGF-IR β-subunit was not detected. However, tyrosine phosphorylation of the intracellular β-subunit was clearly expressed. The results of this study provide basic information on the involvement of IGF system and Vtg during embryonic development of olive flounder(P. olivaceus).
목차
Abstract
Ⅰ. 서론
Ⅱ. 연구방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
References
참고문헌 (27)
참고문헌 신청
Felix G. Ayson / 2002 / Differential Expression of Insulin-like Growth Factor I and II mRNAs during Embryogenesis and Early Larval Development in Rabbitfish, Siganus guttatus / General and Comparative Endocrinology 126(2):165~174
Bailey KM / 2002 / Developmental changes in egg yolk proteins of walleye pollock, Theragra chalcogramma, and a comparative study of immunoreactivity of other North Pacific teleosts and invertebrate eggs / Bull Fish Sci Hokkaido Univ 53:95~105
Bangcaya JP / 2004 / IGF-I, IGF-II and IGF-IR expression as molecular markers for egg quality in mullet and grouper
Giorgi Berishvili / 2006 / Organ-specific expression of IGF-I during early development of bony fish as revealed in the tilapia, Oreochromis niloticus, by in situ hybridization and immunohistochemistry: indication for the particular importance of local IGF-I / Cell and Tissue Research 325(2):287~301
Berwert L / 1995 / Ontogeny of IGF-I and the classical islet hormones in the turbot, Scophthalmus maximus / Peptides 16:113~122
Bailey KM / 2002 / Developmental changes in egg yolk proteins of walleye pollock, Theragra chalcogramma, and a comparative study of immunoreactivity of other North Pacific teleosts and invertebrate eggs / Bull Fish Sci Hokkaido Univ 53:95~105
Bangcaya JP / 2004 / IGF-I, IGF-II and IGF-IR expression as molecular markers for egg quality in mullet and grouper
Giorgi Berishvili / 2006 / Organ-specific expression of IGF-I during early development of bony fish as revealed in the tilapia, Oreochromis niloticus, by in situ hybridization and immunohistochemistry: indication for the particular importance of local IGF-I / Cell and Tissue Research 325(2):287~301
Berwert L / 1995 / Ontogeny of IGF-I and the classical islet hormones in the turbot, Scophthalmus maximus / Peptides 16:113~122
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UCI(KEPA) : I410-ECN-0101-2019-454-000553523