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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.9
- 수록면
- 42 - 49 (8page)
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The suitability of the single cell gel electrophoresis (SCGE) assay as a test for the
monitoring of genotoxicity of aquatic environment was evaluated. The SCGE assay was employed to
detect DNA damage induced in clam (Spisula sachalinensis) exposed to a direct mutagen, N-methyl-
N'-nitro-N-nitrosoguanidine (MNNG) or an indirect mutagen, benzo[a]pyrene (B[a]P). The cells of gill
and digestive glands were isolated from clam by homogenization, which was the optimized cell dissociation
method, and the level of DNA damage was assessed and expressed as mean tail length. In
the gill cells, significant dose- and time-dependent increase was observed in the mean tail length at
the concentration from 0.01 to 0.5 ppm MNNG for 96 h. The linear correlation between relative damage
index (RDI) values was suggested to provide criteria of genotoxicity monitoring for direct acting
mutagen. The dose- and time-dependent responses of the digestive glands cells were less sensitive
than those of the gill cells. In contrast, the genotoxic response resulting from the exposure of
0.01~1.0 ppm B[a]P to clam revealed a higher sensitivity in the digestive glands cells than the gill
cells. The comparison between the time profiles of genotoxic responses in clam and carp, the latter
had been obtained in our previous study, indicated that the metabolism of genotoxic compounds in
the two aquatic organisms were quite different each other. We conclude that the SCGE assay has the
potential as a screening test for routine genotoxicity monitoring of aquatic organisms because of its
higher sensitivity and simplicity.
monitoring of genotoxicity of aquatic environment was evaluated. The SCGE assay was employed to
detect DNA damage induced in clam (Spisula sachalinensis) exposed to a direct mutagen, N-methyl-
N'-nitro-N-nitrosoguanidine (MNNG) or an indirect mutagen, benzo[a]pyrene (B[a]P). The cells of gill
and digestive glands were isolated from clam by homogenization, which was the optimized cell dissociation
method, and the level of DNA damage was assessed and expressed as mean tail length. In
the gill cells, significant dose- and time-dependent increase was observed in the mean tail length at
the concentration from 0.01 to 0.5 ppm MNNG for 96 h. The linear correlation between relative damage
index (RDI) values was suggested to provide criteria of genotoxicity monitoring for direct acting
mutagen. The dose- and time-dependent responses of the digestive glands cells were less sensitive
than those of the gill cells. In contrast, the genotoxic response resulting from the exposure of
0.01~1.0 ppm B[a]P to clam revealed a higher sensitivity in the digestive glands cells than the gill
cells. The comparison between the time profiles of genotoxic responses in clam and carp, the latter
had been obtained in our previous study, indicated that the metabolism of genotoxic compounds in
the two aquatic organisms were quite different each other. We conclude that the SCGE assay has the
potential as a screening test for routine genotoxicity monitoring of aquatic organisms because of its
higher sensitivity and simplicity.
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