Evaluation of Oxidative DNA Damage Using an Alkaline Single Cell Gel Electrophoresis (SCGE) Comet Assay, and the Protective Effects of N-Acetylcysteine Amide on Zearalenone-induced Cytotoxicity in Chang Liver Cells

Changgeun Kang; Hyungkyoung Lee; Yong-San Yoo; Do-Yun Hah; Chung Hui Kim; Euikyung Kim; Jong Shu Kim

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자료유형: 학술저널

저자정보: Changgeun Kang (Gyeongsang National University) Hyungkyoung Lee (Gyeongsang National University) Yong-San Yoo (Gyeongsang National University) Do-Yun Hah (Gyeongnam Livestock Promotion Research Institute) Chung Hui Kim (Gyeongnam National University of Science and Technology) Euikyung Kim (Gyeongsang National University) Jong Shu Kim (Gyeongsang National University)

저널정보: 한국독성학회 Toxicological Research Toxicological Research Vol.29 No.1

발행연도: 2013.3

수록면: 43 - 52 (10page)

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Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium that are found in cereals and agricultural products. ZEN has been implicated in mycotoxicosis in farm animals and in humans. The toxic effects of ZEN are well known, but the ability of an alkaline Comet assay to assess ZEN-induced oxidative DNA damage in Chang liver cells has not been established. The first aim of this study was to evaluate the Comet assay for the determination of cytotoxicity and extent of DNA damage induced by ZEN toxin, and the second aim was to investigate the ability of N-acetylcysteine amide (NACA) to protect cells from ZEN-induced toxicity. In the Comet assay, DNA damage was assessed by quantifying the tail extent moment (TEM; arbitrary unit) and tail length (TL; arbitrary unit), which are used as indicators of DNA strand breaks in SCGE. The cytotoxic effects of ZEN in Chang liver cells were mediated by inhibition of cell proliferation and induction of oxidative DNA damage. Increasing the concentration of ZEN increased the extent of DNA damage. The extent of DNA migration, and percentage of cells with tails were significantly increased in a concentration-dependent manner following treatment with ZEN toxin (p < 0.05). Treatment with a low concentration of ZEN toxin (25 μM) induced a relatively low level of DNA damage, compared to treatment of cells with a high concentration of ZEN toxin (250 μM). Oxidative DNA damage appeared to be a key determinant of ZEN-induced toxicity in Chang liver cells. Significant reductions in cytolethality and oxidative DNA damage were observed when cells were pretreated with NACA prior to exposure to any concentration of ZEN. Our data suggest that ZEN induces DNA damage in Chang liver cells, and that the antioxidant activity of NACA may contribute to the reduction of ZEN-induced DNA damage and cytotoxicity via elimination of oxidative stress.

#Zearalenone #An alkaline single cell gel electrophoresis(SCGE) Comet assay #DNA damaging #Cytotoxicity #N-Acetylcysteine amide(NACA) #Chang liver cell

참고문헌 (45)

참고문헌 신청

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