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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 윤정수
- 발행연도
- 2015
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이 논문의 연구 히스토리 (3)
2015
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PURPOSE: This study is to investigate the effect of acute exercise and recovery on expression of MAPK-actived protein kinase 5 [MAPKAPK5(MK5)] related signaling proteins in mice skeletal muscle.
METHODS: Thirty five male ICR mouse ran the treadmill with the intensity of 15m/min (60% VO₂max) with zero degree. Gastrocnemius muscle exercised after given exercise (15, 60min) and during recovery(6, 12hours). The western blotting performed for the expression of p-MK5, p-RAC1, p-PAK1, p-PAK2, p-ERK3 and p-HSP27 proteins.
RESULTS: The expression of p-Rac1 and p-MK5 was significantly increased during and recovery after exercise. The expression of p-PAK2 and p-MK5 was significantly increased during and recovery after exercise. The expression of ERK3 was significantly increased during exercise (15~60min), and resulting MAPKAPK-5 was also significant during and recovery after exercise. The expression of p-MK5 and p-HSP27 was significantly increased during and recovery after exercise. However, p-PAK1 was significantly decreased during and after exercise and there is no relationship with but MK5 expression.
CONCLUSIONS: The present study can be concluded that p-Rac1, p-PAK2, p-ERK3 and p-HSP27 influenced the p-MK5 expression in mice gastrocnemius muscle. It is suggested that more study needs to clarify the role and signaling pathway of the MK5, especially needs additional experiments using selective inhibitors of MK5.
Therefore, upregulated expression of p-Rac1, p-PAK2, p-ERK3 and p-HSP27 protein after acute exercise showed a similar trend with expressions of p-MK5 in gastrocnemius muscle.
METHODS: Thirty five male ICR mouse ran the treadmill with the intensity of 15m/min (60% VO₂max) with zero degree. Gastrocnemius muscle exercised after given exercise (15, 60min) and during recovery(6, 12hours). The western blotting performed for the expression of p-MK5, p-RAC1, p-PAK1, p-PAK2, p-ERK3 and p-HSP27 proteins.
RESULTS: The expression of p-Rac1 and p-MK5 was significantly increased during and recovery after exercise. The expression of p-PAK2 and p-MK5 was significantly increased during and recovery after exercise. The expression of ERK3 was significantly increased during exercise (15~60min), and resulting MAPKAPK-5 was also significant during and recovery after exercise. The expression of p-MK5 and p-HSP27 was significantly increased during and recovery after exercise. However, p-PAK1 was significantly decreased during and after exercise and there is no relationship with but MK5 expression.
CONCLUSIONS: The present study can be concluded that p-Rac1, p-PAK2, p-ERK3 and p-HSP27 influenced the p-MK5 expression in mice gastrocnemius muscle. It is suggested that more study needs to clarify the role and signaling pathway of the MK5, especially needs additional experiments using selective inhibitors of MK5.
Therefore, upregulated expression of p-Rac1, p-PAK2, p-ERK3 and p-HSP27 protein after acute exercise showed a similar trend with expressions of p-MK5 in gastrocnemius muscle.
목차
Ⅰ. 서 론 11. 연구의 필요성 12. 연구의 목적 63. 연구의 가설 74. 연구의 제한점 8Ⅱ. 이론적 배경 91. 운동과 Conventional MAPKs의 역할 92. Atypical MAPK의 신호전달체계 12Ⅲ. 연구 방법 161. 실험동물 162. 실험방법 171) 실험동물 사육 172) 운동 프로그램 및 근육조직 적출 173) 실험설계 183. 분석 방법 191) Protein 추출 및 western blotting 분석 194. 자료 처리 21Ⅳ. 결 과 221. 일회성 운동 및 운동 후 회복기 비복근 내 phospho MAPKAPK5 protein의 발현 222. 일회성 운동 및 운동 후 회복기 비복근 내 phospho Rac1 protein protein의 발현 233. 일회성 운동 및 운동 후 회복기 비복근 내 phospho PAK1 protein의 발현 244. 일회성 운동 및 운동 후 회복기 비복근 내 phospho PAK2 protein의 발현 255. 일회성 운동 및 운동 후 회복기 비복근 내 phospho ERK3의 발현 266. 일회성 운동 및 운동 후 회복기 비복근 내 phospho HSP27 protein의 발현 27Ⅴ. 논 의 281. 일회성 운동 및 운동 후 회복기에 따른 골격근 내 MK5와 Rac1 및 PAK1/2의 변화 282. 일회성 운동 및 운동 후 회복기에 따른 골격근 내 MK5와 관련된 ERK3 및 HSP27의 변화 32Ⅵ. 결론 및 제언 35참 고 문 헌 37
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