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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.12
- 수록면
- 211 - 216 (6page)
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초록· 키워드
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Dexamethasone (DEX) is a glucocorticoid commonly used to treat idiopathic sudden sensorineural hearing loss (ISSNHL) and inner ear disorders like Meniere's disease. However, systemic administration of DEX is associated with significant side effects, such as hypertension and peptic ulcer, highlighting the need for safer and more effective intratympanic (IT) formulations and reliable methods for their in vivo evaluation. However, methods to determine DEX in the cochlea require a tissue lyser, uncommon in laboratories for instrumental analyses, and their analytical performances have not been validated. To address these issues, a simple and cost-effective method to determine DEX in murine cochlear tissue was developed using triamcinolone acetonide as the internal standard (IS), acetonitrile as a single extraction solvent, and LC-MS/MS as an instrumental method. The developed method was successfully validated through selectivity, linearity (r² ≥ 0.999 within 1–500 ng/mL), accuracy (ranging from 86.8% to 100.2%), precision (≤ 5.8%), matrix effect (91.56% to 104.46%), recovery (93.1% to 104.5%) and the lower limit of quantitation (1.0 ng/mL) following FDA guidelines. This method is expected to contribute to the development of novel formulations for IT delivery of DEX for inner ear disorders.
목차
Abstract
Introduction
Experimental
Results and Discussion
Conclusions
References
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