지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.9
- 수록면
- 544 - 555 (12page)
- DOI
- 10.3349/ymj.2023.0639
이용수
초록· 키워드
오류제보하기
Purpose: By utilizing both protein and mRNA expression patterns, we can identify more detailed and diverse immune cells, providing insights into understanding the complex immune landscape in cancer ecosystems.
Materials and Methods: This study was performed by obtaining publicly available Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) data of peripheral blood mononuclear cells (PBMCs) from the Gene Expression Omnibus database. A total of 94674 total cells were analyzed, of which 32412 were T cells. There were 228 protein features and 16262 mRNA features in the data. The Seurat package was used for quality control and preprocessing, principal component analysis was performed, and Uniform Manifold Approximation and Projection was used to visualize the clusters. Protein and mRNA levels in the CITE-seq were analyzed.
Results: We observed that a subset of T cells in the clusters generated at the protein level divided better. By identifying mRNA markers that were highly correlated with the CD4 and CD8 proteins and cross-validating CD26 and CD99 markers using flow cytometry, we found that CD4+ and CD8+ T cells were better discriminated in PBMCs. Weighted Nearest Neighbor clustering results identified a previously unobserved T cell subset.
Conclusion: In this study, we used CITE-seq data to confirm that protein expression patterns could be used to identify cells more precisely. These findings will improve our understanding of the heterogeneity of immune cells in the future and provide valuable insights into the complexity of the immune response in health and disease.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
Re-defining T-Cell Exhaustion: Subset, Function, and Regulation
Immune Network
2020 .02
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
Absence of Correlation between Changes in the Number of Endothelial Progenitor Cell Subsets
Korean Circulation Journal
2015 .01
-
Single-Cell RNA Sequencing Shows T-Cell Exhaustion Landscape in the Peripheral Blood of Patients with Hepatitis B Virus-Associated Acute-on-Chronic Liver Failure
Gut and Liver
2024 .05
-
Effects of Age and Gender on the Viability and Stem Cell Markers, mRNA, and Protein Expression of Bone Marrow-Derived Stem Cells Cultured in Growth Media
Journal of Korean Dental Science
2018 .12
-
Effect of IL-4 on the Development and Function of Memory-like CD8 T Cells in the Peripheral Lymphoid Tissues
Immune Network
2016 .04
-
Single-cell and spatial sequencing application in pathology
Journal of Pathology and Translational Medicine
2023 .01
-
Analysis of Peripheral B Cell Subsets in Patients With Allergic Rhinitis
Allergy, Asthma & Immunology Research
2018 .01
-
Vascular and immune cell network in the pathogenesis of atherosclerosis
한국실험동물학회 학술발표대회 논문집
2018 .07
-
T-Cells Modified with CD70 as an Alternative Cellular Vaccine for Antitumor Immunity
Cancer Research and Treatment
2020 .01
-
Immune Checkpoint Programmed Cell Death Protein-1 (PD-1) Expression on Bone Marrow T Cell Subsets in Patients With Plasma Cell Myeloma
Annals of Laboratory Medicine
2021 .01
-
Canine regulatory T cells expressing CD4+and CD25+ are proliferated by interleukin-2 cytokine
한국실험동물학회 학술발표대회 논문집
2018 .01
-
Immunological and pathological analyses of peripheral blood mononuclear cell-engraft humanized mouse using NOD-Rag2null IL2rgnull mice
한국실험동물학회 학술발표대회 논문집
2021 .01
-
Effector Memory CD8+ and CD4+ T Cell Immunity Associated with Metabolic Syndrome in Obese Children
Pediatric Gastroenterology, Hepatology & Nutrition
2021 .01
-
Regulation of Th2 Cell Immunity by Dendritic Cells
Immune Network
2016 .02
-
Heterogeneity of Islet Cells during Embryogenesis and Differentiation
Diabetes and Metabolism Journal
2023 .03
-
Effect of Peripheral Blood CD4 + CD25 + Regulatory T Cell on Postoperative Immunotherapy for Patients with Renal Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2016 .01
최근 본 자료
댓글(0)
0