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논문 기본 정보

자료유형
학술저널
저자정보
Furan M. Alp (Department of Agricultural Biotechnology, Faculty of Agriculture, Van Yuzuncu Yil University, Van, Turkey) Genli Gulistan (Department of Agricultural Biotechnology, Faculty of Agriculture, Van Yuzuncu Yil University, Van, Turkey) Yıldız Mehtap (Department of Agricultural Biotechnology, Faculty of Agriculture, Van Yuzuncu Yil University, Van, Turkey) 선현진 (Subtropical Horticulture Research Institute, Jeju National University, Jeju 63243, Korea)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports Vol.18 No.4
발행연도
2024.8
수록면
551 - 561 (11page)
DOI
10.1007/s11816-024-00915-3

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ITS sections, which are situated inside the ribosomal DNA operon, are recognized for their high levels of diversity and slow rate of evolution, which makes them important for plant phylogenetic studies. The objective of this work is to use the internal transcribed spacer (ITS) regions of 18 alfalfa (Medicago sativa) genotypes/varieties to determine their genetic relationships and to verify possible genetic markers for variety identifcation and classifcation. This has been done by validating the sec- ondary structures of the ITS regions and observing the efects of the relationships among the lowest energy state, the total number of possible secondary structure hairpins, and (GC) content. The results indicated that the length of the ITS sequences varied between 426 and 629 nucleotides and that the G + C content of the ITS region varied between 46.6 and 50%. In this study, the analysis of molecular variance (AMOVA) was used to validate the efectiveness of confguration analysis and revealed that fuctuations in the data accounted for a substantial amount of the observed total variance. Moreover, 94 percent of the genotypes analyzed exhibited a signifcant degree of variety. This shows that a large fraction of the observed variation is likely attributable to genetic variables. Population four, comprising Bilensoy80, Emily, Quin, Vendor, and Felicia, had the highest degree of heterozygosity at 68.8%, whereas population two, including Gozlu1, Prosementi, Nimet and Local (Van), had the lowest level at 37.5%. The original Shannon’s approach as an unbiased estimator employed in population genetics research validated the diferences among alfalfa genotypes with the AMOVA analysis results of this study. Individual dif- ferences were found to be 59%, whereas population diferences were found to be 41%. There have been a few ITS studies on Medicago sativa that have utilized ITS as a phylogenetic marker to estimate connections and defne new taxonomic categories (e.g., tribes). However, our research also includes an analysis of the secondary structure of these sequences and the results of this study imply that ITS sequence and secondary structure data can be utilized to understand the intraspecifc genetic makeup of diferent alfalfa varieties.

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