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자료유형
학술저널
저자정보
Rahman, Md. Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
저널정보
한국초지조사료학회 한국초지조사료학회지 한국초지조사료학회지 제36권 제3호
발행연도
2016.1
수록면
243 - 247 (5page)

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Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

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