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논문 기본 정보

자료유형
학술저널
저자정보
Chen Wenwen (Biological Science Research Center Southwest University) Wang Genhong (Biological Science Research Center Southwest University) Yi Meiqin (Biological Science Research Center Southwest University) Gao Tianke (Biological Science Research Center Southwest University) Song Chunman (R&D Center of China Tobacco Yunnan Industrial Co. Ltd) Gao Qian (R&D Center of China Tobacco Yunnan Industrial Co. Ltd) Xia Qingyou (Biological Science Research Center Southwest University) Xiang Haiying (R&D Center of China Tobacco Yunnan Industrial Co. Ltd)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제3호
발행연도
2023.6
수록면
389 - 399 (11page)
DOI
10.1007/s11816-023-00828-7

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Cytokinin hormones are indispensable for plant growth and development. Cytokinin oxidase/dehydrogenase (CKX) helps regulate the dynamic balance of endogenous cytokinin levels. However, little is known about the CKX genes of Nicotiana tabacum (NtCKXs). In silico analyses were used to isolate, characterize, and elucidate the phylogenetic relationships of 15 NtCKX genes. Multi-species phylogenetic tree construction placed NtCKX1–15 on five of the eight branches of the CKX phylogenetic tree. Protein structure and network analyses revealed that NtCKX genes located on the same phylogenetic branch generally contained several conserved motifs and have highly similar structures, with structural domains related to flavin adenine dinucleotide (FAD) and cytokinin-binding found on all of the predicted NtCKX-encoded proteins. The upstream promotor region of NtCKX genes contained many abiotic stress-responsive cis-acting elements, including DRE, ERE, MBS, MYB, and MYC. Gene expression analysis revealed that each NtCKX gene responded differently to salt stress and exogenous abscisic acid (ABA) treatment. Four NtCKX genes exhibited ABA-induced expression trends with varying peak times. Under salt stress, NtCKX expression was significantly suppressed in two genes and upregulated in five others. In summary, we provided basic information regarding the CKX gene family of N. tabacum and elucidated their gene expression patterns under abiotic stresses, including ABA treatment and salt stress. The findings of this work can serve as a foundation for future study of the functions of NtCKX genes.

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