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논문 기본 정보

자료유형
학술저널
저자정보
Arif Soukaina (Moulay Ismail University of Meknès, Laboratory of Biotechnology and Bioresources Valorization, BP 11201, Zitoune Meknes City, MoroccoMoulay Ismail University of Meknès, Cluster of Competency) M’Barek Hasna Nait (Moulay Ismail University of Meknès, Cluster of Competency «Agri-food, Safety and Security» IUC VLIR-UOS, Marjane 2, BP 298, Meknes City, MoroccoParis-Saclay University, CentraleSupélec, Euro) Bekaert Boris (Ghent University, Faculty of Bioscience Engineering, Department of Plants and Crops, Valentin Vaerwyckweg 1, Schoonmeersen - gebouw C 9000 Ghent, Belgium) Aziz Mohamed Ben (Sultan Moulay Sliman University, Higher School of Technology, Laboratory of Biotechnology, Bioresources, and Bioinformatics (3BIO), 54000 Khenifra, Morocco) Diouri Mohammed (Moulay Ismail University of Meknès, Laboratory of Biotechnology and Bioresources Valorization, BP 11201, Zitoune Meknes City, MoroccoMoulay Ismail University of Meknès, Cluster of Competency) Haesaert Geert (Ghent University, Faculty of Bioscience Engineering, Department of Plants and Crops, Valentin Vaerwyckweg 1, Schoonmeersen - gebouw C 9000 Ghent, Belgium) Hajjaj Hassan (Moulay Ismail University of Meknès, Laboratory of Biotechnology and Bioresources Valorization, BP 11201, Zitoune Meknes City, MoroccoMoulay Ismail University of Meknès, Cluster of Competency)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.5
발행연도
2024.5
수록면
1,017 - 1,028 (12page)
DOI
10.4014/jmb.2312.12048

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초록· 키워드

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Lignocellulolytic enzymes play a crucial role in efficiently converting lignocellulose into valuable platform molecules in various industries. However, they are limited by their production yields, costs, and stability. Consequently, their production by producers adapted to local environments and the choice of low-cost raw materials can address these limitations. Due to the large amounts of olive stones (OS) generated in Morocco which are still undervalued, Penicillium crustosum, Fusarium nygamai, Trichoderma capillare, and Aspergillus calidoustus, are cultivated under different fermentation techniques using this by-product as a local lignocellulosic substrate. Based on a multilevel factorial design, their potential to produce lignocellulolytic enzymes during 15 days of dark incubation was evaluated. The results revealed that P. crustosum expressed a maximum total cellulase activity of 10.9 IU/ml under sequential fermentation (SF) and 3.6 IU/ml of β-glucosidase activity under submerged fermentation (SmF). F. nygamai recorded the best laccase activity of 9 IU/ml under solid-state fermentation (SSF). Unlike T. capillare, SF was the inducive culture for the former activity with 7.6 IU/ml. A. calidoustus produced, respectively, 1,009 μg/ml of proteins and 11.5 IU/ml of endoglucanase activity as the best results achieved. Optimum cellulase production took place after the 5th day under SF, while ligninases occurred between the 9th and the 11th days under SSF. This study reports for the first time the lignocellulolytic activities of F. nygamai and A. calidoustus. Furthermore, it underlines the potential of the four fungi as biomass decomposers for environmentally-friendly applications, emphasizing the efficiency of OS as an inducing substrate for enzyme production.

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