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논문 기본 정보

자료유형
학술저널
저자정보
Ryu Han-bin (Department of Biological Engineering, Inha University, Incheon 22212, Republic of KoreaIndustry-Academia Interactive R) Kang Mi-Jin (Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea) Choi Kyung-Min (Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea) Yang Il-Kyu (Department of Biological Engineering, Inha University, Incheon 22212, Republic of KoreaIndustry-Academia Interactive R) Hong Seong-Joo (Department of Biological Engineering, Inha University, Incheon 22212, Republic of KoreaIndustry-Academia Interactive R) Lee Choul-Gyun (Department of Biological Engineering, Inha University, Incheon 22212, Republic of KoreaIndustry-Academia Interactive R)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.2
발행연도
2024.2
수록면
407 - 414 (8page)
DOI
10.4014/jmb.2311.11046

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Phosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis sp. with inhibited polyphosphate degradation and the effect of this engineered Synechocystis biomass on lettuce growth. First, a strain (phoU) lacking the phoU gene, which encodes a negative regulator of environmental phosphate concentrations, was generated to inhibit polyphosphate degradation in cells. Polyphosphate concentrations in the phoU knock-out strain were maintained for 24 h and then decreased slowly. In contrast, polyphosphate concentrations in the wild-type strain increased up to 4 h and then decreased rapidly. In addition, polyphosphate concentration in the phoU knockout strain cultured in semi-permeable membrane bioreactors with artificial wastewater medium was 2.5 times higher than that in the wild type and decreased to only 16% after 48 h. The biomass of lettuce treated with the phoU knockout strain (0.157 mg P/m2 ) was 38% higher than that of the lettuce treated with the control group. These results indicate that treating lettuce with this microalgal biomass can be beneficial to crop growth. These results suggest that the use of polyphosphate-accumulating microalgae as biofertilizers may alleviate the effects of a diminishing phosphorous supply. These findings can be used as a basis for additional genetic engineering to increase intracellular polyphosphate levels.

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