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논문 기본 정보

자료유형
학술저널
저자정보
Seok-Eun Lee (Department of Preventive Dentistry College of Dentistry Dankook University Cheonan) Ja-Won Cho (Dankook University) 조자원 (단국대학교) 유현준 (단국대학교) Myung-Gu Lee (Department of Biomedical Chemistry College of Biomedical and Health Science Konkuk University Chungju Korea) Yeol-Mae Jeon (Dankook University) 김다희 (단국대학교 보건과학대학 치위생학과) Hye-Won Park (Department of Preventive Dentistry College of Dentistry Dankook University Cheonan)
저널정보
대한예방치과학회 International Journal of Clinical Preventive Dentistry International Journal of Clinical Preventive Dentistry Vol.17 No.3
발행연도
2021.9
수록면
117 - 127 (11page)

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Objective: This study aimed to investigate the antimicrobial activity and cytotoxicity of zinc oxide eugenol (ZOE)-based and mineral trioxide aggregate (MTA)-based root canal sealers. Methods: The root canal sealers used in this study were Tubli-Seal (a ZOE-based sealer) and ProRoot (an MTA-based sealer). The antimicrobial activity of ProRoot and Tubli-seal was assessed using a susceptibility assay using Enterococcus faecalis, Porphyromonas endodontalis, and Porphyromonas gingivalis. The cytotoxicity of the root canal sealers was investigated using MTT assay, real-time RT-PCR, and ELISA after the pulp cells were treated with sealers. Additionally, the components of the root canal sealers were analyzed using X-ray fluorescence. Results: The MTA-based sealer showed stronger antimicrobial activity against E. faecalis than the ZOE-based sealer, and the antimicrobial activities of both sealers against P. endodontalis and P. gingivalis were not significantly different. The ZOE-based sealer showed strong cytotoxicity towards pulp cells and induced the expression and production of inflammatory cytokines. However, the MTA-based sealer did not affect the viability of pulp cells or significantly induce the expression of inflammatory cytokines such as TNF-α, IL-6, and IL-8. In the component analysis, the MTA-based sealer had more kinds of metal oxides than the ZOE-based sealer. Conclusion: The MTA-based sealers had stronger antimicrobial activity against E. faecalis compared to the ZOE-based sealer, and did not show significant cytotoxicity towards pulp cells. Based on these results, MTA-based sealers may be a suitable material for root canal therapy.

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