Direct Monitoring of Membrane Fatty Acid Changes and Effects on the Isoleucine/Valine Pathways in an ndgR Deletion Mutant of Streptomyces coelicolor

Choi Tae-Rim; Oh Suk Jin; Hwang Jeong Hyeon; Kim Hyun Jin; Shin Nara; Yun Jeonghee; Lee Sang-Ho; Bhatia Shashi Kant; Yang Yung-Hun

doi:10.4014/jmb.2301.01016

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자료유형: 학술저널

저자정보: Choi Tae-Rim (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of Korea) Oh Suk Jin (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of Korea) Hwang Jeong Hyeon (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of Korea) Kim Hyun Jin (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of Korea) Shin Nara (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of Korea) Yun Jeonghee (Department of Forest Products and Biotechnology Kookmin University Seoul 02707 Republic of Korea) Lee Sang-Ho (Department of Pharmacy College of Pharmacy Jeju National University Jeju-si 63243 Republic of Korea) Bhatia Shashi Kant (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of KoreaInstitute for Ubiquitous Information Technology and Applications Konkuk University Seoul 05029) Yang Yung-Hun (Department of Microbial Engineering College of Engineering Konkuk University Seoul 05029 Republic of KoreaInstitute for Ubiquitous Information Technology and Applications Konkuk University Seoul 05029)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제6호

발행연도: 2023.6

수록면: 724 - 735 (12page)

DOI: 10.4014/jmb.2301.01016

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NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.

#Streptomyces coelicolor A3(2) #ndgR #phospholipid fatty acid #gas chromatography-mass spectrometry

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